HPLC法测定柴丹疏利胶囊中丹参素的含量

Content Determination of DanShenSu in ChaiDan ShuLi Capsule with HPLC

目的:建立HPLC法测定柴丹疏利胶囊中丹参素含量的方法。方法:采用反相高效液相色谱法,使用Agilent HC-C18 Analytical(5μm,4.6mm×250mm)色谱柱,以甲醇∶冰醋酸∶水(4∶1∶95)为流动相,流速1.0 mL/min,检测波长281nm。结果:丹参素在11.3～226μg/mL范围内线性关系良好,其线性方程为y=13 010x+10 419(r=0.999 9)。丹参素的平均回收率为99.40%,RSD=0.30%(n=6)。结论:该方法简便、可靠、准确,可作为该制剂的质量控制方法。

Objective： Toestablishthemethodfor content determinationof DanShenSu inChaiDan ShuLi capsule with HPLC.Method： RP-HPLC method was adopted.Agilent HC-C18 Analytical column（5μm,4.6 mm×250 mm） was used with mobile phase consisted of methanol-glacial acetic acid-water（4：1：95） at flow rate of 1.0 mL/ min.The detection wave length was 281 nm.Result： There was a good linear relationship in the range of 11.3-226 μg/ mL, the linear equation is y=13 010x ＋10 419（r=0.999 9）.Average recovery rate was 99.40%,RSD=0.30%（n=6）.Conclusion： The method is simple,reliable,accurate and could be applied to quality control of the preparation.