两种肺炎支原体快速检测方法的比较研究

Comparative study on two methods of fast detection of Mycoplasma Pneumoniae

目的研究肺炎支原体(MP)快速鉴定培养基法及胶体金免疫斑点法检测MP抗体法,在快速诊断MP感染中的临床价值。方法选择解放军306医院呼吸科病房522例急性呼吸道感染病人,分别取同一患者咽拭子样本用快速鉴定培养基法进行MP培养,并取血清用胶体金免疫斑点法进行MP抗体检测,对两种方法的结果做出评价。结果两种方法的检测结果有较好的一致性。受检患者中,136例MP快速培养法为阳性,阳性率为26.1%;而123例用胶体金免疫斑点法检测MP抗体为阳性,阳性率为23.6%;两种检测结果之间差异无统计学意义(P=0.105,P>0.05)。结论应用此两种方法快速诊断感染MP均较方便、快速,但快速培养法能在发病早期就得到结果,有更好的临床应用价值。

Objective To explore the clinical value of Mycoplasma pneumoniae （MP） rapid culture method and Dot Immun- gogold method （DIM） for fast detection of MP infection. Methods It took 522 patients with acute respiratory infection in respiratory ward of Chinese PLA 306th Hospital as samples. For every patient, his pharynx swabs were taken for fast culture with MP and his serum was obtained for detection of MP antibody with Dot Immungogold method. Then the two methods were compared based on the results. Results The two methods had good consistency, which were： 26.1% of 136 patients had positive results by the MP rapid culture method, and 23. 6% of 123 patients had positive results by DIM. The difference between them had no statistical significance （P = 0. 105, P 〉 0.05 ）. Conclusions The two methods of fast detection of MP infection are convenient and fast, but the method of rapid culture can obtain result in early onset of the disease, which has better clinical value.