大鼠运动病模型—条件性厌食症的建立

Establishment of conditioned taste aversion as a motion sickness model in rats

目的 建立大鼠条件性厌食症两种实验模型 ,并进行初步的药物抗运动病应用试验。方法 ( 1)给 SD大鼠禁水 ,观察旋转刺激前及刺激后连续 4d各 2 4h内对 0 .15 %糖精水的饮用量 ,了解旋转对大鼠饮糖精水行为的影响及持续时间。 1周后用同批大鼠重复实验 1次。 ( 2 )给大鼠禁水 2 4h,然后测量各鼠 45 m in内 0 .15 %糖精水的饮用量。观察旋转刺激前后的变化及药物的影响。结果 方法( 1)中 ,不受旋转刺激的动物 ,连续 3d每天的糖精水饮用量无明显变化。而旋转刺激后 ,前 3d糖精水的饮用量明显减少 ,第 4天时恢复。 1周后重复实验结果相同。方法 ( 2 )亦证明旋转刺激使大鼠产生对糖精水的厌饮行为 ,生姜、半夏等六味草药煎剂能促进大鼠糖精水的饮用。结论 结果表明旋转刺激使大鼠产生条件性厌食症。厌饮表现可持续 3d,并且同一批动物可重复实验。生姜、半夏等六味草药煎剂可抑制由方法 ( 2 )引起的条件性厌食症。因此 ,用旋转刺激引起的条件性厌食症是较好的运动病模型 。

Objective To establish two experimental models of conditioned taste aversion(CTA) in rats and to test the antimotion sickness effects of some drugs.Methods (1) Before and 1~4 d after rotatory stimualtion,the volume of intake of 0.15% saccharin solution every 24 h was measured in rats with fast of water,and this experiment was repeated using the same rats a week later;(2) 0.15% saccharin solution was given the rats to drink after 24 h fast of water,then the volume intake in 45 min was measured before and 1 d after the rotatory stimulation.At the same time,the influences of certain drugs on the intake behavior of saccharin solution of the rats were observed.Results In method (1),without rotatory stimulation,the rat′s intake of saccharin solution showed no change in 3 days.However,after the rotatory stimulation,the intake volume was shown reduced for 3 days and then recovered in the fourth day.Such taste aversion behavior was observed again with repeated experiment 1 week later and also in the method (2),experiment.The Chinese medical herb solution decocted from ginger,pinellia and other four herbs had shown increased the intake of saccharin solution.Conclusions [WT5”,6BZ]The taste aversion to saccharin solution after rotatory stimulation is a CTA,which is a manifestation of motion sickness.This taste aversion behavior could maintain for 3 days and this animal model could be used repeatedly.Decocted ginger compound juice could inhibit the CTA induced by method II.The authors maintain that CTA is a good model of motion sickness and both above two methods could be used as needed.