无症状HBsAg携带者血液、尿液和唾液中前S1抗原检测分析

DETECTION AND ANALYSIS OF THE PRE S1 ANTIGEN IN THE BLOOD,URINE AND SALIVA OF ASYMPTOMATIC HBsAg CARRIERS

目的 :探讨前S1抗原在无症状HBsAg携带者血液、尿液和唾液中的存在情况及其与其它指标的相互关系。方法 :收集 73例无症状HBsAg携带者的血液、尿液和唾液标本各 73份 ,采用ELISA法检测HBsAg、HBeAg、前S1抗原和前S2抗原 ;用斑点杂交法测HBV -DNA。结果 :前S1抗原在血液、尿液和唾液中的检出率分别为 5 8 9%、16 4%和 10 9% ,可以和HBeAg及前S2指标互相替换 ,血清中前S1抗原检出率高于尿液和唾液标本。结论 :前S1抗原可在无症状HBsAg携带者的血液、尿液和唾液中检出 ,是反映乙型肝炎传染性的一项很有意义的指标。

Objectives:To study the presence of Pre S1 antigen in the blood,urine and saliva of asymptomatic HBsAg carriers and the relation to the other signs Methods:The 73 blood,urine and salive were collected from 73 asymptomatic HBsAg carriers respective ly;HBsAg,HBeAg,Pre S1 antigen and Pre S2 antigen were detected with ELISA kit,and HBV-DNA with nucleic acid hybridization Results:The detectable rate of Pre S1 antigen were 58 9%,16 4% and 10 9% in the three types samples respectively HBeAg and Pre S2 antigen could be alternated by Pre S1 antigen The positive rate of Pre S1 antigen was higher in blood than in urine and in saliva Conclusions:(1)The Pre S1 antigen can be detected in the blood,urine and saliva of asymptomatic HBsAg carriers;(2)The Pre-S1 antigen may be a good marker to indicating viral replication and infectivity of HBV infection