抗DNP抗体检测及其临床应用

ANTIBODY AGAINST DNP DETECTING AND THE CLINICAL SIGNIFICANCE

目的 :介绍一种可直接检测红斑狼疮因子 (抗DNP抗体 )的简便方法及其临床意义探讨。方法 :用胶乳免疫法检测血清中的抗DNP抗体 ,同时对同一受检者进行红斑狼疮细胞 (LEC)检查 ,比较两种方法的检出率 ,并分析与疾病的关系。结果 :LEC阳性者抗体均可检出阳性 ;在 12 1例SLE中 ,LEC阳性 19例 ,阳性率 15 7% ,抗DNP抗体阳性 31例 ,阳性率 2 5 6 % ,为LEC的 1 6倍 ;抗DNP抗体阳性者均为SLE活动期患者 ,占SLE活动期患者的 6 0 9% ;抗DNP抗体的出现与否与抗ds-DNA抗体水平相关 ,抗ds-DNA抗体≥ 2 +时出现抗DNP抗体阳性 ,抗ds-DNA抗体≤ 2 +时 ,抗DNP抗体检为阴性 ;发现 8例患者治疗后抗DNP抗体分别在 2 3、34、38、48、6 2、85、2 0 1、32 3天后转阴 ,并伴随有抗ds -DNA抗体水平的下降。结论 :检测抗DNP抗体即用免疫的方法直接检测血清中红斑狼疮因子 ,可替代红斑狼疮细胞 (LEC)检查 ,操作简便、快速 ,检出率高。

Objective:To introduce a simple method of detecting a kind of lupus erythematosus cell factor (LEC,antibody against DNP)directly and to discuss the clinical significance of this method Methods:Serum anti DNP was tested with latex immunoassay and at the same time lupus erythenatosus cell(LEC)of the same patient was detected Then we compared the detection rates of both methods and analyzed the relationships between the detection results and the disease of SLE Results:To people whose LEC were positive,their anti-DNP antibodies were all positive Even no missed detection occurred In 121 cases of SLE,the LEC results of 19 cases were positive with a positive rate of 15 7% and the anti-DNP results of 31 casecs were positive with a positive rate of 25 6%,which was 1 6 times as high as LEC results All the cases with positive anti-DNP were patients of SLE in active stage Whether anti-DNP appeared was associated with the level of antibody against ds-DNA When the level of anti-ds-DNA≥2+,anti-DNP was positive While anti-ds-DNA≤2+,anti-DNP was negtive In addition,anti-DNP of 8 cases were detected negative 23,34,38,48,62,85,201,323 days after treatment,repectively,with the decline of anti-ds-DNA Conclusion:Detecting serum anti-DNP directly with immunoassay was a simple,fast method with high detection rate and low missed detection rate It could take the place of the detection of LEC