重复超排技术在纯种和牛核心群建立中的应用

Establishing of Nucleus Herd of Purebred Wagyu by the Repeated Superovulation

为了快速扩繁纯种和牛核心群,以最大限度发挥其繁殖潜力。试验以南北方3个典型地区的纯种和牛为试验对象,采用CIDR+FSH+PG方法进行超数排卵,探索合理的重复超排次数及此技术在不同地域环境下的应用状况。高强度重复超排试验表明:初次超排获得的可用胚胎数最理想,随着超排次数的增加,可用胚胎数量有所降低,但是差异不显著(P>0.05);地域间不同超排间隔的超排数据比较,不同地区超排效果差异不显著(7.38±4.27)、(7.88±5.95)、(7.45±5.24)(P>0.05)。在良好的饲养管理前提下,高强度重复超排以1个月为超排间隔,超排3～4次为宜;纯种和牛的适应能力较强,超排间隔在1—2个月,超排3次的常规超排方案可以应用于南北方地区和牛核心群的建立。

In order to establish the core group of the purebred Wagyu and maximize their reproductive potential,which from three typical regions in South and North have been superovulated using the method of CIDR ＋ FSH ＋ PG,to explore the reasonable number of repeated superovulation and applied status in the different environment.The results showed that：the experiment of high strength repeated superovulation showed that the most of the transferable embryos have been recovered in the first superovulation,the increased superovulated times have a negative influence on the number of transferable embryos,however,the influence was not significant（P0.05）;there were no significant differences among different regions for the transferable embryos（7.38±4.27,7.88±5.95,7.45±5.24,P0.05）.In good breeding management,the suitable number of superovulation times were 3 to 4 when the interval was 1 month for high strength repeated super ovulation.The conventional superovulated program,which have 1 to 2 months interval and 3 times could be used to establish the core group of purebred Wagyu in our country.