大叶南五味子多溶剂分步提取物的抗菌活性研究

Antibacterial Activity of Different Solvent Separate-step Extract from Kadsura marmorata in Vitro

为了探讨大叶南五味子果实的体外抗菌活性。将大叶南五味子依次以石油醚、乙酸乙酯、无水乙醇,水为溶媒,每种溶剂均先用超声波提取20min,再加热回流提取2h,采用微管-平板法测定4种溶剂的提取物对鸡大肠杆菌、猪链球菌和金黄色葡萄球菌的最小抑菌浓度(MIC)。结果显示:有机溶媒提取率在0.35%～5.50%,水提取率达到51.2%;有机溶媒提取物对鸡大肠杆菌、猪链球菌、金黄色葡萄球菌的MIC为0.44～34.08mg/mL,水提取物对鸡大肠杆菌、猪链球菌、金黄色葡萄球菌的MIC分别为204.63、329.13、232.5mg/mL。说明大叶南五味子果实有机溶媒提取物虽然得率少,但其化学成分具有较高的抗菌活性。该研究为大叶南五味子临床应用、抗菌活性成分的分离奠定基础。

In order to explore antibacterial activity of Kadsura marmorata fruit in vitro.Kadsura marmorata which solved in petroleum ether,ethyl acetate,anhydrous ethanol and water in turn,was extracted 20 min by ultrasonic extraction,then 2 h with a hot reflux,respectively.The minimal inhibitory concentration（MIC） of four extracts was detected against chicken E.coli,Streptococcus Suis and Staphylococcus aureus with the microtubule-plate method.The results showed that extraction rates of organic solvent were 0.35%-5.50% and water was 51.2%.MIC of organic solvent extracts reached 0.44-34.08 mg/mL against chicken E.coli,Streptococcus Suis and Staphylococcus aureus,while MIC of water extract was 204.63 mg/mL,329.13 mg/mL,232.5 mg/mL against chicken E.coli,Streptococcus Suis and Staphylococcus aureus respectively.This study indicated that the Kadsura marmorata fruit extraction of organic solvent had lower yield,but higher antibacterial activity elements than extraction of water.This study laid the foundation for clinical application and separation of antibacterial activity components from Kadsura marmorata.