低值水产品蛋白资源酶解产物的抗氧化性分析和评价

Antioxidant Analysis and Evaluation of Protease-digested Productions for two Kinds of Low-valuable Fish Proteins

为分析评价水产品蛋白酶解产物活性特性及合适水解蛋白酶类的选择。采用不同的商品化蛋白酶和枯草蛋白酶BPN对市场上常见的经济鱼类白鲢和彩云雕蛋白进行酶解,测定各种酶在水解过程中的水解度,并对酶解产物的抗氧化活性进行了比较分析。结果表明:蛋白酶在水解反应的前20min内水解度迅速增加,20～120min之间逐渐达到平衡。复合蛋白酶和水解蛋白酶具有较强的水解能力,其中水解蛋白酶对彩云雕2h的水解产物最高可达15.92%。白鲢鱼枯草蛋白酶水解液的抗氧化活性最好,其DPPH清除能力为87.11%。不同蛋白酶水解不同的水产品蛋白产生的酶解产物活性肽成分具有明显的差异。

This experiment aimed to evaluate and study hydrolysis capacities of different commercial proteases on two kinds of low-valuable fish and activities of the resultant protease-digested productions.The results showed that the hydrolysis capacities of the tested proteases increased rapidly within first 20 min,and then began to a flatten curve out or declining in 2 hours.The complex protease dialogue showed the higher hydrolysis capacity on silver carp,and also with the biggest 15.92% hydrolysis degree of commercial hydrolysis protease on Red Tilapia.Hydrolysis products of subtilisin BPN on silver carp showed strongest antioxidant activity in all the enzyme-digested products,up to 87.11% ability to clear the DPPH.We concluded that the activities of the hydrolysis products were very different in different commercial proteases on two kinds of fish proteins.