高效液相色谱法测定谷胱甘肽滴眼液中苯扎氯铵的含量

Determination of Benzalkonium Chloride in Glutathione Eye Drops by HPLC

目的:建立测定谷胱甘肽滴眼液中苯扎氯铵含量的方法。方法:采用AgilentZorbaxSB-CN柱(4.6mm×250mm,5μm);流动相:0.1%磷酸水溶液-乙腈-四氢呋喃(52∶38∶10);检测波长:214nm。结果:苯扎氯铵的线性范围为50～250μg/ml,线性回归方程为Y=20674X-79747(r=0.99979)。平均回收率分别为100.9%,RSD为0.19%。结论:该方法简便、快速、准确,具有良好的重复性和回收率,可作为该制剂的质量控制标准。

Objective：To establish a HPLC method For the determination of Benzalkonium Chloride in Glutathione eye drops. Methods：The sample was analyzed on an Agilent Zorbax SB-CN column（4.6mm×250mm,5μm） with mobile phase consisting of 0.1% phosphoric acid and acetonitrile and tetrahydrofuran（52∶38∶10） and the detection wavelength was 214nm. Results：The Calibration curves of Benzalkonium Chloride was linear in the range of 50～250μg/ml and the linear equation was Y=20674X-79747（r=0.99979）.The average recoveries was 100.9% with RSD 0.19%. Conclusion ：This method is simple, rapid, accurate and with good repeatability and recovery, it cab be used as a quality control method for this preparation.