马铃薯StHb1蛋白的亚细胞定位

Subcellular localization of StHb1 protein from potato

为了研究马铃薯StHb1蛋白的亚细胞定位情况,采用RT-PCR技术克隆到马铃薯StHb1基因cDNA序列,并成功构建了StHb1基因与绿色荧光蛋白基因的融合表达载体pBI121-StHb1-GFP.利用农杆菌介导法将重组载体转化洋葱内表皮细胞,通过荧光显微镜观察融合蛋白的瞬时表达以确定StHb1蛋白在细胞内的分布.结果表明StHb1蛋白主要分布于细胞核中.

To identify the subcellular location of StHb1 protein,a cDNA encoding nonsymbiotic hemoglobin（StHb1） was cloned from Solanum tuberosum by RT-PCR,and fused to the 5′ end of the green fluorescent protein gene to generate plant binary vector pBI121-StHb1-GFP.It was introduced into onion epidermal cells via Agrobacterium-mediated transformation.Subcellular localization of StHb1 protein was detected by fluorescent microscopy.The results showed that the StHb1-GFP fusion proteins were predominantly present in the nucleus.