苦石莲的抗肿瘤化学成分研究

Antitumor constituents from seeds of Caesalpinia minax

目的研究苦石莲的抗肿瘤活性成分。方法采用柱色谱技术对苦石莲的提取物进行分离纯化,采用波谱技术结合化学方法进行结构鉴定。结果从苦石莲提取物中分离得到了12个化合物,包括8个卡山烷型二萜类化合物,分别鉴定为neocaesalpin A(1)、neocaesalpin B(2)、neocaesalpin L(3)、caesalpinin MD(4)、neocaesalpin MA(5)、neocaesalpin H(6)、neocaesalpin P(7)、chagreslactone(8)、β-香树素(9)、β-谷甾醇(10)、胡萝卜苷(11)和没食子酸(12)。结论化合物8为首次从云实属植物中分离得到,化合物4～7为首次从该植物中分离得到,化合物1和4具有一定的抑制肿瘤细胞增殖作用。

Objective To study the antitumor constituents in the seeds of Caesalpinia minax.Methods The constituents were isolated and purified by repeated silica gel column chromatography and the structures were identified by spectral analyses and chemical methods.Results Twelve compounds including eight cassane-type diterpenes were isolated from the seeds of C.minax and their structures were identified as neocaesalpin A（1）,neocaesalpin B（2）,neocaesalpin L（3）,caesalpinin MD（4）,neocaesalpin MA（5）,neocaesalpin H（6）,neocaesalpin P（7）,chagreslactone（8）,β-amyrin（9）,β-sitosterol（10）,daucosterol（11）,and gallic acid（12）.Conclusion Compound 8 is first obtained from the plants in Caesalpinia L.Compounds 4—7 are first obtained from C.minax and compounds 1 and 4 show moderate cytotoxic activities in inhibition of tunmor proliferation.