附子总生物碱对乳腺癌小鼠的抗肿瘤作用

Antitumor effect of total alkaloids from Aconiti Lateralis Radix Praeparata on breast cancer in mice

目的观察附子总生物碱对二甲基苯蒽诱导的乳腺癌小鼠体征、部分血液学指标及肿瘤的影响。方法小鼠分为对照组、模型组、附子总生物碱组。模型组小鼠每天sc二甲基苯蒽橄榄油溶液50 mg/kg,每周2次、连续5周,建立乳腺癌模型,给药组在每天给予二甲基苯蒽的同时,给予附子总生物碱2 mg/kg,观察实验期间各组小鼠乳腺肿瘤的潜伏期、发生率、外观、体温和外耳微循环变化,检测血清中雌二醇和孕酮水平、红细胞Na+,K+-ATP酶和Ca2+,Mg2+-ATP酶活性及血液流变学指标,综合评价附子总生物碱对小鼠乳腺癌的影响。结果模型组小鼠随给药时间的延长逐渐出现畏寒喜暖、踡缩少动、体温下降、外耳微循环受阻状况,同时血中雌二醇和孕酮水平升高,红细胞ATP酶活性降低,全血黏度及红细胞聚集指数增高;附子总生物碱可以显著改善上述指标变化,阻止肿瘤生长。结论二甲基苯蒽诱导的乳腺癌小鼠表现为体寒血瘀体征,附子总生物碱能改善这些症状,阻止肿瘤进展。

Objective To investigate the effects of total alkaloids from Aconiti Lateralis Radix Praeparata（TAALRP） on the manifestation of breast cancer induced by 7,12-dimethylbenzanthracene（DMBA）,some hematological indexes,and tumor growth in mice.Methods The mice were divided into control,model,and TAALRP groups.Mice in the model group were sc injected with DMBA（50 mg/kg in olive oil） twice weekly for five weeks to establish a breast cancer model;Mice in TAALRP group were simultaneously ig administrated with TAALRP（2 mg/kg）.The changes in tumorigenic latency,incidence,appearance,body temperature,and auricle microcirculation were observed in carcinogenic process;The levels of estradiol and progesterone in serum,the activities of erythrocytic Na＋,K＋-ATPase and Ca2＋,Mg2＋-ATPase,and hemorrheologic indexes were detected.The effects of TAALRP on breast cancer in mice were comprehensively evaluated.Results The mice in the model group appeared gradually aversion to cold and preference for warmth,laziness and drowsiness,body temperature dropping,and auricle microcirculation disorder as the administration time prolonged.Meanwhile,the estradiol and progesterone levels in serum increased,while erythrocytic ATPase activity decreased.However,the whole blood viscosity and erythrocytic aggregation index increased.TAALRP showed a reduction in changed indexes and inhibited tumor growth.Conclusion The mice with breast cancer induced by DMBA appear a manifestation of body coldness with blood stasis.The treatment with TAALRP could improve the symptoms and signs,and prevent the tumor growth.