刺五加提取物对果蝇免疫功能的影响

Effect of extract from roots and rhizomes of Acanthopanax senticosus on immune function of Drosophila melanogaster

目的研究刺五加提取物对果蝇天然免疫和肠道免疫功能的影响。方法用含或不含刺五加根或果实提取物的细菌或真菌生物溶液感染果蝇,观察刺五加根和果实提取物对感染细菌或真菌以及对热刺激果蝇生存率的影响;果蝇肠道组织免疫染色,荧光显微镜观察肠壁细胞凋亡情况;提取被真菌孢子感染的果蝇总RNA,检测抗菌肽表达量。结果刺五加根和果实提取物能提高细菌感染后果蝇的生存率,缓解肠壁细胞凋亡,也可提高经真菌孢子感染后果蝇的生存率,使体内部分抗菌肽出现高表达,也能提高经热刺激处理果蝇的生存率。结论刺五加根和果实提取物能显著提高果蝇机体免疫功能。

Objective To study the effect of the extract from the roots and rhizomes of Acanthopanax senticosus（ERA） and extract especially here from the fruits of of A.senticosus（EFA） on innate immunity and gut immunity of Drosophila melanogaster.Methods D.melanogaster was infected by bacterial or fungal biological solution with or without ERA or EFA,and the effects of ERA or EFA on the survival rates of D.melanogaster with microbial infection and thermal stimulation were recorded.Cell apoptosis was observed using fluorescence microscopy after immunostaining gut tissue of D.melanogaster.The total RNA of spore-infected D.melanogaster was extracted and the expression of antimicrobial peptides was detected.Results ERA and EFA could improve the survival rate of bacteria-infected D.melanogaster,meanwhile alleviate the apoptosis of gut epithelial cells.The survival rate of spore-infected D.melanogaster was improved by ERA and EFA,and certain antimicrobial peptides were highly expressed in vivo.ERA and EFA could also improve the survival rate of D.melanogaster with thermal stimulation.Conclusion ERA and EFA could significantly improve the immune function of D.melanogaster.