丹参注射液对中重度IUA患者宫内膜αvβ3、PDGF及TIMP-1的影响

Effects of danshen injection on αvβ3,PDGF and TIMP-1 in endometrium of patients with moderate to severe intrauterine adhesions

目的宫腔注入丹参注射液防治中重度IUA,观察αvβ3、PDGF及TIMP-1在治疗前后宫内膜的表达变化,探讨其与IUA的关系,进一步阐明丹参注射液防治IUA的作用机理。方法将90例中重度IUA患者随机分为丹参组、欣可聆组及空白组,每组30例,采用双向电泳方法,观察治疗前后αvβ3、PDGF及TIMP-1的表达情况。结果治疗前3组αvβ3、PDGF及TIMP-1的表达水平比较差别无统计学意义(P>0.05),治疗后丹参组及欣可聆组宫内膜PDGF及TIMP-1表达水平低于空白组(P<0.05),而αvβ3的表达高于空白组(P<0.05),丹参组及欣可聆组比较差别无统计学意义(P>0.05)。结论αvβ3、PDGF及TIMP-1的异常表达导致子宫纤维增殖活跃,可能参与宫腔粘连的发生发展,丹参注射液及欣可聆均能减少粘连形成,提高子宫内膜容受性。

Objective To observe the changengs of αvβ3,PDGF and TIMP-1 before and after therapy,by infusing danshen injection into uterine cavity,through such a way to investigate the relationship between danshen injection and IUA,and to elucidate the mechanism of this medicine in preventing and treating IUA.Methods 90 patients were randomly divided into danshen group,viscoelastic solution group and blank control group,30 patients in each group.Observing the changes of αvβ3,PDGF and TIMP-1 before and after therapy,through two-dimensional electrophoresis.Results Three groups had no difference in αvβ3,PDGF and TIMP-1 before therapy（P0.05）,while after therapy the PDGF and TIMP-1 expression in blank control group were more than the other two groups（P0.05）,but the αvβ3 level was less than the other two groups（P0.05）.There were no difference between viscoelastic solution group and danshen group in αvβ3,PDGF and TIMP-1.Conclusion The abnormal expression of αvβ3,PDGF and TIMP-1can proliferate uerine fiber,and participate in development and progression of IUA,danshen injection and viscoelastic solution can reduce IUA rate and increase endometrial receptivity through regulating expression of αvβ3,PDGF and TIMP-1.