电场对低温保存生物组织介电特性影响的研究

Effect of Electric Field on the Dielectric Properties of Biological Tissue under Cryopreservation

以SD大鼠的肾、皮肤、肝脏、大脑皮层、心脏和肌肉为研究对象,在对其进行慢速冻结的过程中施加外电场,研究了电场对生物组织低温保存介电特性的影响。结果表明:不同条件下6种离体生物组织的阻抗均随着频率的升高而减小,介质损耗因数随着频率的升高先增大后减小;在相同频率下,电冷冻保存的阻抗和介质损耗因数介于刚离体新鲜组织与直接冷冻保存的阻抗和介质损耗因数之间。阻抗越大,损耗越低,组织新鲜度和完整性越高,说明电冷冻保存离体组织的新鲜度和完整性比直接冷冻有不同程度的提高。

Using the kidney, skin, liver, cerebral cortex, heart and muscle of SD rats as research object, the effects of electric field on the dielectric properties of SD tissues under cryopreservation were studied by applying external electric field in their slow rate freezing process. The results show that with the increase of frequency, the impedance of six kinds of biological tissue decreases; the dielectric loss factor increases firstly and then decreases. Under the same frequency, the impedance and dielectric loss factor of the biological tissue preserved by electro-freezing are between that of the fresh tissue and direct cryopreservation. The greater is the impedance, the lower the loss is, and the better the freshness and integrity are, indicating that the electro-freezing can improve the freshness and integrity of tissues to some extents compared to the direct cryopreservation.