摘要
探讨苦瓜总皂苷(TSMC)对糖尿病大鼠肾脏保护作用及其作用机制。将Wistar大鼠随机分为4组,A:正常对照组;B:模型组;C:300mg/kg苦瓜总皂苷组;D:100mg/kg苦瓜总皂苷组。灌胃给药每日1次,8周后,检测各组肾组织中空腹血糖、肌酐(Cr)、尿素氮(BUN);放免分析检测血清胰岛素(Ins)、α1微球蛋白(α1-MG)、β2微球蛋白(β2-MG)。RT-PCR检测肾组织血管内皮生长因子(VEGF)水平的变化。与糖尿病组相比,苦瓜总皂苷治疗组肌酐、尿素氮、α1-MG、β2-MG显著降低(P<0.05),VEGF mRNA的表达减弱(P<0.05)。苦瓜总皂苷可显著降低糖尿病大鼠组织VEGF mRNA的表达,改善肾功能,延缓糖尿病肾病发展。
To investigate the effect and mechanism of total saponins from Momordica charantia（TSMC）on renal function in diabetic rats.Wistar rats were randomly divided into diabetic model group,diabetic model treated with 300mg/kg TSMC,100mg/kg TSMC and normal control group.The animals were intra-gastriclly administered with corresponding drugs once daily.After 8 weeks,the fasting blood glucose（FBG）,blood urea nitrogen（BUN）and creatinine（Cr）were measured,and serum levels of insulin（Ins）,α1-MG and β2-MG was determined by radioimmunoassay.RT-PCR were used to detect the expressions of vascular endothelial growth factor（VEGF）mRNA in the renal tissue of rats.The levels of α1-MG,β2-MG,BUN,Cr and the mRNA Expression of VEGF in TSMC group were lower than that of diabetic model（P0.05）.TSMC could obviously improve renal function and decrease the mRNA expression of VEGF in kidney tissue,and delay the progression of diabetic nephropathy in diabetic nephropathy model rats.
出处
《中国食物与营养》
2012年第9期73-75,共3页
Food and Nutrition in China
关键词
苦瓜总皂苷
糖尿病肾病
血管内皮生长因子
total saponins from Momordica charantia
diabetic nephropathy
vascular endothelial growth factor（VEGF）
