猕猴桃^(60)Co-γ射线辐射诱变育种适宜剂量的研究

Search for proper dose of ^(60)Co-γ ray in Actinidia chinensis radiation breeding

以果肉红色的‘红阳’猕猴桃、果肉黄色‘桂海4号’猕猴桃和果肉绿色的‘长果’猕猴桃的枝条为试材,用4种剂量的60Co-γ射线辐射处理,以未照射枝条为对照,采用随机区组设计进行田间嫁接。对嫁接成活率、植株生长量的调查分析。结果显示:在25～150Gy辐射剂量范围内,随辐射剂量的加大,对嫁接成活率及生长的抑制作用逐渐增强。不同品种的猕猴桃对辐射的敏感性差异较大。3个品种猕猴桃辐射半致死剂量分别为50.6Gy(红阳)、64.4Gy(桂海4号)、71.7Gy(长果猕猴桃)。试验结果可以为猕猴桃辐射育种适宜剂量的确定提供依据。

In this experiment red flesh Actinidia chinensis ‘ HongYang ’, yellow flesh A.chinensis ‘ GuiHai No. 4 ’ and green flesh A.chinensis ‘ ChangGuo ’ branches were treated with 60^Coγray in dose of 25Gy , 50Gy , 100 Gy and 150Gy respectively and cutivated in randomized blocks design after treatment.The untreated branches used as control.Surivial rate and height of plant were examined.The result indicated that it was obviously that the inhibition of both the survival rate and the height of plant would be enhanced with increase of the radi- ation dose ranged in this experiment.Different varieties of A.chinensis quite different sensitivity to radiation. The medial lethal dose of 60^Co-γradiation for these three varieties of A.chinensis branches were 50.6Gy （ Hong Yang ）、 64.4Gy （ GuiHai NO.4 ）、 71.7Gy （ Chang Guo ） .The results would provide an important experimental basis for the radiation breeding of other varieties of A.chinensis.