摘要
对葡萄溃疡病菌菌丝摇培时间、酶解时间、渗透压稳定剂种类及再生培养基类型进行了探索和优化,建立了葡萄溃疡病菌稳定、高效的原生质体制备体系。以SR培养基作为再生培养基时,原生质体再生率达到最高,为32.2%。该研究为建立葡萄溃疡病菌高效、稳定的遗传转化体系,开展其致病机理的研究奠定了基础。
The culture time of mycelium,the enzymolysis time,the type of osmotic stabilizer and the regeneration medium were optimized in this study.An efficient method for protoplast preparation and regeneration of Botryosphaeria rhodina was obtained.While SR medium was chosen as the regeneration medium,the regeneration rate of protoplast reached the highest,up to the maximum of 32.2%.This study provided powerful support for genetic transformation and transformant pathogenicity analysis in Botryosphaeria spp.
出处
《植物保护》
CAS
CSCD
北大核心
2012年第5期27-30,63,共5页
Plant Protection
基金
国家葡萄产业技术体系(CARS-30)
