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数字显微全息中颗粒重建数值模拟

Numerical simulation of particle reconstruction in digital holographic microscopy
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摘要 采用数值模拟方法研究了颗粒空间位置及间距对显微全息测量结果(颗粒位置和粒径)的影响,分别考察了颗粒在平行和垂直光轴方向上,电荷耦合元件(CCD)平面内不同间距和不同粒径情况下的颗粒重建结果,讨论了灰度阈值对颗粒识别的影响,并结合实验对实际拍摄得到的颗粒全息图进行重建分析.结果表明:在垂直光轴方向上,当记录距离分别为5、50、100μm时,可以识别出粒径均为1μm的双颗粒的最小间距分别对应为1、2、2μm;在平行光轴方向上,记录距离分别为5、50、100μm时,可以识别出粒径均为1μm的双颗粒的最小间距分别对应为2、8、16μm;在重建过程中,灰度阈值应设定为不小于0.5. The influence of particle position and distance on the results of measurement （positioning and di- ameter measurement） was investigated. Reconstruction results of particles in the plane of Charge-coupled Device （CCD） with different distances and diameters in parallel with optical axis and perpendicular to opti- cal axis were studied, respectively. The influence of gray threshold on particle recognition was discussed. Experimental hologram of particles were also reconstructed and analyzedl Results showed that the mini- mum recognized distances perpendicular to optical axis of two particles with 1 tam in diameter were 1 μm, 2 μm, 2 μm, related to the recording distances of 5 μm, 50 μm, 100 μm, respectively. The minimum rec- ognized distances in parallel with optical axis of two particles with 1 μm in diameter were 2 μm, 8 μm, 16 μm, related to the recording distances of 5 μm, 50 μm, 100 μm, respectively. The gray threshold should be no smaller than 0.5 in the process of reconstruction.
作者 曲瑞陽 吴学成 高翔 吴迎春 陈玲红 G.Grehan 岑可法
机构地区 浙江大学能源清洁利用国家重点实验室 法国国家科研中心CNRS 鲁昂大学 法国国家应用科学研究院鲁昂分院CORIA研究所
出处 《浙江大学学报（工学版）》 EI CAS CSCD 北大核心 2012年第9期1647-1653,共7页 Journal of Zhejiang University：Engineering Science
基金 国家自然科学基金资助项目(51176162,51125025) 国家“973”重点基础研究发展规划资助项目(2009CB219802) 高等学校学科创新引智计划资助项目(B08026)
关键词 微流场 数字显微全息 颗粒间距 灰度阈值 误判率 micro-scale flow field digital holographic microscopy particle distance gray threshold un-recognition rate
分类号 TK11 [动力工程及工程热物理—热能工程]
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参考文献30

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浙江大学学报（工学版）
2012年 第9期

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