川楝素对人肝癌细胞株HepG2生物学行为的影响

Effent of toosendanin on the biological behavior of human hepatoma cell line HepG2

目的观察川楝素对人肝癌细胞株HepG2增殖和凋亡的影响，并研究其诱导凋亡的机制。方法取人肝癌HepG2细胞株培养后，随机分为对照组（不添加任何药物）、观察组（加川楝素80mmol／m1），培养细胞24、48、72h后，酶标仪测定人肝癌HepG2细胞增殖抑制率；流式细胞仪检测人肝癌HepG2细胞周期和凋亡率。结果两组对肝癌HepG2细胞增殖抑制率差异有统计学意义（χ2=5．33，P〈0．05）；两组S期、G0／G1期、M／G2：期的细胞比率（t=6．31、6．26、6．56，均P〈0．05）、细胞凋亡率差异有统计学意义（χ2=6．15，P〈0．05）。结论川楝素可明显抑制人肝癌细胞HepG2的恶性增殖，并诱导肿瘤细胞凋亡。

Objective To observe the effect of toosendanin on proliferation and apoptosis of human hepatoma cell line HepG2 cells, and to study the mechanism. Methods Human hepatocellular carcinoma HepG2 cells were ran- domly divided into control group（ group A） does not add any medication,the observation group（ group B） joined TSN 80mmol/ml, cultured cells for 24,48,72 hours. HepG2 cells proliferation inhibition rate was measured by MTT;Hepa- tocellular carcinoma HepG2 cell cycle and apoptosis rate was deteded by flow cytometry. Results TECAN determina- tion revealed two groups of hepatocellular carcinoma cell proliferation had statictical significance （ χ2 = 5.33, P 〈 0. 05）;flow cytometry revealed two groups S, G0/G1 ,M/G2 phase of the cell rate（t = 6.31,6.26,6.56, all P 〈 0. 05）, apoptosis rate had significant difference （ χ2 = 6.15, P 〈 0.05 ）. Conclusion Toosendanin could obviously inhibit the proliferation of HepG2 cells, and induce apoptosis of tumor cells.