连翘子总酚的树脂纯化工艺优选

Optimization of Purification Technology for Total Phenol from Seed of Forsythia suspensa by Macroporous Resin

目的:探索连翘子总酚的大孔吸附树脂纯化工艺。方法:以连翘子总酚的吸附量和解吸率为指标,从AB-8,CAD-40,HPD-300,D-101型大孔吸附树脂中筛选最佳型号;以连翘子总酚得量为指标,采用单因素试验考察树脂用量、水洗用量、洗脱溶剂的种类和用量对连翘子总酚纯化工艺的影响;以连翘子总酚得量为指标,选取吸附流速、洗脱流速及树脂径高比为考察因素,采用正交试验优选连翘子总酚大孔树脂纯化工艺。结果:AB-8型树脂的纯化效果最佳,其优选的纯化工艺为树脂用量2倍生药量,树脂径高比1∶5,吸附流速0.25 BV.h-1,加5 BV水洗除杂,用2 BV 80%乙醇洗脱,洗脱流速1.0 BV.h-1。在此工艺条件下连翘子总酚的纯度达65.56%。结论:优选的总酚纯化工艺稳定可行,为连翘子中总酚类成分的工业化生产提供实验依据.

Objective： To explore purification technology of total phenol from seed of Forsythia suspense by macroporous adsorption resin. Method： With adsorption capacity and desorption ratio of total phenol from seed of F. suspense as indexes, optimum resin model was screened from AB-8, CAD-40, HPD-300 and D101 macroporous resin; With yield of total phenol from seed of F. suspense as index, effect of four factors on purification technology for total phenol from seed of F. suspense was investigated by single factor test, such as the amount of resin and water, kind and amount of elution solvent; With yield of total phenol from seed of F. suspense as index, adsorption flow rate, elution flow rate and resin diameter-high ratio were selected as factors, purification technology of total phenol from seed of F. suspense was optimized by orthogonal test. Result： Experiment result showed that AB-8 resin possessed optimal purification effect, its optimized purification technology was as follows： resin-crude drug 2： 1, diameter-high ratio 1 ： 5, adsorption flow rate 0.25 BV.h-1, washed by 5 BV water to impurity, eluted with 2 BV 80% ethanol, elution flow rate 0.25 BV.h-1. Under these technology conditions, purity of total phenol from seed of F. suspense was up to 65.56%. Conclusion： Optimized purification technology of total phenol was stable and feasible, it provided evidence for industrial production of total phenol from seed of F. suspense.