摘要
目的:建立RP-HPLC测定辣蓼药材中槲皮素的的含量,测定10个产地辣蓼中槲皮素的含量。方法:HypersilBDS C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.4%磷酸(50∶50)等度洗脱,流速1 mL.min-1,室温,检测波长365nm,进样量20μL。结果:槲皮素标准曲线回归方程为A=73 185C-21.47(r=0.999 5),在0.004～0.014 g.L-1线性关系良好,平均回收率为99.29%,RSD 1.30%。结论:所用方法分离效果好,简便、准确,可用于辣蓼药材的质量控制。
Objective： To determine the quercetin content of Polygonum flaccidum from different growing areas. Method： The chromatographic column was Hypersil BDS C18 （4.6 mm × 250 mm, 5 μm）. The mobile phase was methanol-0.4% phosphoric acid （50： 50）, The flow rate was 1 mL ·min^（-1） , the detection wavelength was at 365 nm. The column temperature was at room temperature. The sample volume was 20 p,L. Result： The standard curve regression equation was A =73 185C-21.47 （r =0. 999 5）. It showed a good linear relationship between 0. 004-0. 014 g·L^（-1） , and average recovery was 99.29%, RSD 1.30%. Conclusion： The method is simple and reliable, could be used to control the quality of these medicinal materials.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第21期89-91,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科学研究与技术开发计划项目(桂科能10100027-1)
