HPLC测定小儿清肺化痰颗粒中黄芩苷的含量

Determination of Baicalin in Xiaoerqingfeihuatan Granule by HPLC

目的:建立高效液相色谱法测定小儿清肺化痰颗粒中黄芩苷的含量方法。方法:Agilent XDB C18色谱柱(4.6 mm×250 mm,4.6μm);流动相:甲醇-0.4%磷酸溶液(47∶53);流速1.0 mL.min-1;检测波长280 nm柱温:40℃。结果:回归方程为A=34 884.9C-19 478.8,R2=1.000 0。黄芩苷在1.482～148.2 mg.L-1线性关系良好,平均回收率为97.8%,RSD 1.9%(n=6)。结论:该方法简便、准确、重复性好,可作为小儿清肺化痰颗粒黄芩苷含量的测定。

Objective： To establish a method for the determination of baicalin in Xiaoerqingfeihuatan granule by HPLC. Method： HPLC was used on an Agilent XDB Cls （4.6 mm ×250 ram, 4.6 μm） column with acetonitrile-0.4% phosphoric acid （47： 53） as mobile phase. The flow rate was 1.0 mL ·min^（-1） with detection wavelength at 280 nm and column temperature at 40℃. Result： The regression equation of baicalin is A = 34 884.9C - 19 478.8, R2 = 1. 000 0 （n =6）. The linear range of baicalin was from 1. 482-148.2 g · L^（-1）. The average recovery was 97.8% , and RSD was 1.9% （ n = 6）. Conclusion ： The method is simple, fast and accurate with good reproducibility. It can be used for the determination of Xiaoerqingfeihuatan granule.