GC-MS法研究东北刺人参挥发油抗真菌活性成分

Study on the Antifungal Active Constituent of Essential Oil from Oplopanax elatus by GC-MS

目的:研究东北刺人参挥发油中抗真菌活性成分。方法:按新版中国药典中水蒸气蒸馏法提取东北刺人参挥发油,采用试管内药基法研究其抗真菌活性,利用GC-MS分析其抗真菌活性成分。结果:东北刺人参挥发油抗真菌活性MIC为0.034%～0.063%,MFC为0.070%～0.126%,挥发油中水溶性成分共18个,已鉴定其中12个化合物,占挥发油总量的90.71%,其中萜烯醇类化合物占89.83%,橙花叔醇占38.40%,毕橙茄醇占28.44%。结论:东北刺人参挥发油中水溶性成分抗真菌活性更显强,主要化学成分为橙花叔醇和毕橙茄醇。

Objective： To study the antifungal active constituent of essential oil from Oplopanax elatus. Method ： The essential oil of Oplopanax elatus was steam-distilled according to the China Pharmacopoeia to extract. Antifungal activity studies were carried out in vitro and antifungal active constituent were analyzed by GC-MS. Result： Antifungal active MIC of essential oil from Oplopanax elatus ranged from 0. 034%-0. 063% and MFC 0.070%-0. 126%. 18 components were obtained from water-soluble essential oil and 12 components were identified. 89.83% of them were terpenol compounds, nerolidol （ 38.40% ） and T-cadinol （ 28.44% ）Conclusion： Antifungal activity of water-soluble essential oil from Oplopanax elatus was strong. The main components were nerolidol and T-cadinol.