HPLC测定癃闭舒片中补骨脂素和异补骨脂素的含量

Determination of Psoralen and Isopsoralen in LBSP by HPLC

目的:应用高效液相色谱法测定癃闭舒片(LBSP)中补骨脂素和异补骨脂素的含量。方法:C18色谱柱(4.6 mm×150 mm,5μm),甲醇-0.02 mol.L-1磷酸二氢钾水溶液(45∶55)为流动相,流速1.0 mL.min-1,检测波长246 nm。结果:补骨脂素在0.144 0～0.720 0μg进样量与峰面积呈良好的线性关系(r=0.999 8),平均加样回收率为99.16%,RSD1.21%(n=6);异补骨脂素在0.136 2～0.681 0μg进样量与峰面积呈良好的线性关系(r=0.999 8),平均加样回收率为98.52%,RSD 1.58%(n=6)。结论:该方法测定结果准确、灵敏、重复性好。

Objective： To develop an HPLC method for determination of the content of psoralen and isopsoralen in Longbishu pills （LBSP）. Method： An C18 column was used as the chromatographic column. The mobile phase consisted of methanol-0.02 mol·L^（-1） Sodium Dihydrogen Phosphate Solution （45： 55） , the flow rate was 1.0 mL · min^（-1） The UV detection wavelength was at 246 nm. Result： There was a good linear relationship between the concentration of psoralen and peak area value when the concentrations of psoralen Was within the range of 0. 144 0-0. 720 0 Ixg （r =0. 999 8）. The average recovery was 99. 16% , RSD 1.21% （n =6）. There was a good linear relationship between the concentration of isopsoralen and peak area value when the concentrations of isopsoralen was within the range of 0. 136 2-0. 681 0 μg （r =0. 999 8）. The average recovery was 98.52% , RSD 1.58% （n = 6）. Conclusion： The method was accurate, sensitive, reproducible and may be used in the determination of psoralen and isopsoralen in LBSP.