基于液质联用技术的HIV/AIDS患者尿液代谢组学研究

Research on Urine Metabonomics of HIV/AIDS Patiens Based on HLPC-MS Technique

目的:探寻艾滋病及人类免疫缺陷病毒携带(HIV/AIDS)患者尿液的代谢组变化,探讨代谢组学方法在预防和延缓HIV病毒感染者病程进展研究中的应用前景。方法:运用液质联用技术(LC-MS),检测55例HIV/AIDS患者和20例健康人的尿液代谢成分,结合主成分分析(PCA)和t检验分析技术,分析HIV/AIDS患者尿液的特异性代谢物质(或群谱)。结果:HIV/AIDS患者组与健康对照组经代谢组学的方法在尿液代谢轮廓上得到了良好区分,发现了10个可能存在的潜在生物学标记物。结论:代谢组学的研究方法可以将HIV/AIDS患者与健康人进行良好的区分,综合代谢标记物变化趋势构成了HIV/AIDS的代谢特征,代谢组学的方法在预防和延缓HIV病毒感染者病程进展的研究中将有很好的应用前景。

Objective： To investigate human immunodeficiency virus （HIV/AIDS） in urine of patients with metabolic changes and to explore future application value of metabonomics technique in research of preventing and delaying the acquired immunodeficiency syndrome. Method： Using liquid chromatography coupled to mass spectrometry （LC-MS） , urine metabolic components in 55 HIV/AIDS patients and 20 healthy human beings were studied by metabonomics method, and combined with principal component analysis （PCA） and t test analysis technology, the specific metabolic substances （or group spectrum） were analyzed. Result： The urine metabolic profiles in the two groups showed differences with 10 potential biological markers. Conclusion： From the metabonomics method, HIV/AIDS patients and healthy people can be well differentiated. Comprehensive metabolic markers indicate HIV/AIDS metabolic characteristics. Metabonomics approach in the study of prevention and delay of HIV virus infection will have good prospects.