栀子苷的快速提取分离及其镇痛抗炎作用研究

Fast Extraction of Geniposide and its Analgestic and Anti-inflammatory Effect

目的:快速提取分离栀子苷单体,研究其体内镇痛、抗炎作用。方法:大孔吸附树脂初步纯化后,采用中压柱层析系统从中药栀子中快速提取分离栀子苷单体。将SPF级BALB/c小鼠分为5组,分别为对照组(生理盐水),栀子苷50,100,200 mg.kg-1组,吗啡或阿司匹林组(5,100 mg.kg-1)。小鼠尾静脉注射一次给药后,采用醋酸扭体实验、热板实验、二甲苯耳肿胀实验,角叉菜胶足肿胀实验,评价分离得到的栀子苷单体的体内镇痛、抗炎作用。结果:大孔吸附树脂初步纯化后得到栀子苷含量为78.96%,中压柱层析纯化后,栀子苷单体含量提高到98.47%。与对照组比较后得到,提取出的栀子苷能显著减少由腹腔注射醋酸引起的小鼠扭体次数(P<0.05),抑制二甲苯引起的耳肿胀(P<0.05)以及角叉菜胶引起的足肿胀(P<0.05),并呈现剂量依赖性。对小鼠热板痛阈值无明显影响。结论:实现了栀子苷的快速制备分离,分离获取的栀子苷具有较好的体内镇痛、抗炎作用。

Objective： To extract and separate the geniposide monomer rapidly, and study its analgesic and anti-inflammatory effects. Method： Macroporous resin was used to rich and isolate geniposide from Fruit Gardenia jiaminoides and medium pressure column chromatography system was used to purify geniposide monomer quickly. BALB/c mice were divided into 5 groups： normal control group （saline） , geniposide groups （50, 100, 200 mg ·kg-1） and morphine group or aspirin group （5, 100 mg ·kg-1）. Administrated by tail vein injection, hot plate test, acetic writhing test, xylene ear swelling test and the carrageenan paw edema test were used to evaluate the analgesic and anti-inflammatory activities of geniposide monomer in mice. Result： The content of geniposide was raised to 78.96% with macroporous resin, and 98.47% with medium-pressure column chromatography purification. Geniposide monomer could significantly reduce the writhing actions, which were induced by the intraperitoneal injection of acetic acid in mice, inhibit the xylene-induced ear swelling and carrageenan-induced paw swelling with a dose-dependent manner （P 〈 0.05）, but it could not increase the hot plate pain threshold. Conclusion： Geniposide was separated and purified rapidly and successfully and it exhibited good analgesic and anti-inflammatory effects in mice.