凉血活血方对腹腔感染脓毒症大鼠肠组织纤溶及胶原代谢的影响

Effects of Liangxue Huoxue Formula on Fibrinolytic Function and Collagen Metabolism of Intestinal Tissue in Septic Rats

目的:研究凉血活血方对腹腔感染脓毒症大鼠肠组织纤溶及胶原代谢的影响,探讨其作用机制。方法:Wistar大鼠50只,雌雄各半,随机分为假手术组、模型组、凉血活血方治疗组。模型组大鼠以丝线结扎盲肠末端,针头穿孔,挤出少许粪便后关腹,制作腹腔感染脓毒症模型。治疗组于术后开始给予活血凉血方进行ig治疗,每日1次,连续治疗72 h,假手术组和模型组给予等体积蒸馏水ig。统计动物死亡率,ELISA法测定存活大鼠肠组织的组织纤溶酶原激活物(t-PA),Ⅰ型纤溶酶原激活物抑制因子(PAI-1),结缔组织生长因子(CTGF),成纤维细胞生长因子-10(FGF-10),Ⅰ型胶原,Ⅲ型胶原含量或活性。结果:凉血活血方治疗组动物死亡率为25%,与模型组(65%)比较显著降低(P<0.05);与假手术组相比,模型组t-PA水平显著降低(P<0.01),而PAI-1,CTGF,FGF-10,I型胶原,III型胶原显著升高(P<0.01);与模型组相比,凉血活血方治疗组t-PA水平显著回升(P<0.01),而PAI-1,CTGF,FGF-10,Ⅰ型胶原,Ⅲ型胶原水平显著降低(P<0.01)。结论:凉血活血方具有良好的纤溶调节、抗胶原增生作用,对腹腔感染脓毒症具有良好的治疗作用。

Objective ： The present work was designed to study the effects of Liangxue Huoxue formula on fibrinolytic function and collagen metabolism of intestinal tissue in septic rats. Method： Fifty Wistar rats w^ere divided into control, model and treatment group randomly. Sepsis model was established by cecal ligation and puncture （CLP） in rats. Rats in treatment group were administered orally with Liangxue Huoxue formula liquid extract once daily for 72 hours, while those in control and model group with same amount of distilled water. The survival rate was measured. The concentrations of tissue-type plasminogen activator （t-PA）, plasminogen activator inhibitor-1 （PAI-1）, connective tissue growth factor （CTGF）, fibroblast growth factor-10 （FGF-IO）, type I collagen and type IU collagen in intestinal tissue were determined by ELISA method. Result： Liangxue Huoxue formula decreased the mortality to 25% , much lower than that in model group （P 〈0.05）. Compared with control group, the t-PA level was decreased （P 〈0.01） while the expressions of PAI-1, CTGF, FGF-10, type I collagen and type III collagen in intestinal tissue were increased （P 〈 0.01 ） in model group. Liangxue Huoxue formula increased t-PA level （P 〈 0.01 ） and reduced the expressions of PAI-1, CTGF, FGF-10, type I collagen and type III collagen in intestinal tissue significantly （P 〈 O. O1 ）. Conclusion： The fibrinolytic function and collagen metabolism play an important role in the pathological process of multiple organ dysfunction in sepsis and Liangxue Huoxue formula has therapeutic effect on sepsis nmdel by regulating the fibrinolytic function and collagen metabolism.