侗族药羊耳菊醇提物抗炎镇痛作用的实验研究

Experimental Study on Anti-inflammatory and Analgesic Effects of Ethanol Extracts of Dong-medicine Inula cappa

目的:观察侗族药羊耳菊醇提物的抗炎镇痛作用。方法:小鼠分为空白对照组,羊耳菊醇提物(生药)2.5,5 g.kg-1剂量组和阳性对照阿司匹林0.25 g.kg-1组。采用二甲苯致小鼠耳肿胀法、小鼠腹腔毛细血管通透性亢进法观察其抗炎作用;采用小鼠醋酸扭体法、热板法观察其镇痛作用。结果:羊耳菊醇提物(5 g.kg-1)显著抑制二甲苯所致小鼠耳肿胀(P<0.05)及醋酸所致小鼠腹腔毛细血管通透性增高(P<0.01);羊耳菊醇提物(5 g.kg-1)显著减少醋酸致小鼠20 min内扭体次数,提高热板致痛小鼠痛阈值(P<0.01,P<0.01)。结论:羊耳菊醇提物具有较好的抗炎镇痛作用。

Objective： To observe anti-inflammatory and analgesic effects of ethanol extracts of Dong- medicine Inual cappa. Method： Mice divided into blank control group, low dose group and high dose group （2.5, 5 g·kg^（-1）） of ethanol extracts of I. cappa DC. and positive control group of aspirin （0.25 g ·kg ^（-1））. The anti-inflammatory action was assessed by tests including HAc-induced peritoneal capillary permeabihty in mice, xylene-induced ear edema in mice. The analgesic effect was verified by HAc-induced writhing response in mice and hot-plate test. Result： The dose group of the ethanol extracts of I. cappa DC. （5 g ＂kg-1） could significantly inhibit the ear edema induced by xylene （P 〈 0.05 ） and very significantly inhibit the phlogistic infiltration induced by acetic acid in mice （P 〈0.01）. The dose group of the ethanol extracts of lnual cappa DC. （5 g ＂kg-L） could significantly reduced writhes of abdominal cavity induced by acetic acid in 20 minutes and increased the pain threshold in the hot-plate test （P 〈 0.01, P 〈 0.01 ）. Conclusion： Ethanol extracts of Inual cappa DC. have obvious anti-inflammatory and analgesic effects.