SFE-GC/HPLC对重离子辐照育种的玉米油中五种营养物质的含量分析

ANALYSIS OF FIVE NUTRIENTS CONTENTS IN THE OIL OF MAIZE BRED BY HEAVY ION IRRADIATION BY SFE-GC/HPLC METHOD

目的分析重离子辐射育种的玉米油中棕榈酸、油酸、亚油酸、维生素E(VE)和β-胡萝卜素5种营养物质的含量变化。方法重离子辐照育种的玉米粒粉碎后利用CO2-SFE萃取技术进行玉米油的提取分离,然后利用GC色谱和HPLC色谱分别对棕榈酸、油酸和亚油酸及VE和β-胡萝卜素进行含量的检测分析。结果重离子辐照处理对玉米油中5种营养物质含量的积累具有正、负双重效应,对于多数辐照品种营养物质的含量基本呈现增加趋势,增加的范围分别为4.35%～18.39%、5.21%～28.73%、3.545%～12.18%、0.5587%～28.02%和7.621%～19.46%;但对于某些辐照品种,营养物质的积累也会表现为下降,比如,CSR24001的油酸含量相对于对照降低了7.21%,金象4C-1的VE含量相对于对照降低了14.52%;除CSR24001外,辐照后3种脂肪酸总量均有不同程度增加,其中,棕榈酸所占比例因品种差异而不同、油酸除CSR24001外均有所增加、亚油酸则均表现为下降。结论通过重离子辐照进行玉米诱变育种,可有效提高玉米油中营养物质含量,改变营养物质的比例,进而改进玉米籽粒的品质。

Objective To analyze the changes of the contents of palmitic acid,oleic acid,linoleic acid,vitamin E（VE） and β-carotene in the oil of maize bred by heavy ion irradiation.Method The maize oil was extracted and separated by CO2-SFE firstly and then the contents of palmitic acid,oleic acid,linoleic acid and VE,β-carotene were identified by GC and HPLC respectively.Results The contents of five above nutrients in the oil of maize bred by heavy ion irradiation was increased 4.35%-18.39%,5.21%-28.73%,3.545%-12.18%,0.5587%-28.02% and 7.621%-19.46% respectively,compared to that bred without heavy ion irradiation.But the contents as oleic acid of CSR24001 and VE of Jinxian4C-1 was decreased 7.21% and 14.52% respectively.The total amount of palmitic acid,oleic acid and linoleic acid was increased to some extent except CSR24001.The ratio of palmitic acid was different with the species.The oleic acid was increased except CSR24001 and the linoleic acid decreased in all species.Conclusion The nutrients contents in the oil of maize bred by heavy ion irradiation could be effectively improved and the ratio of nutrient could be changed.Thereby it could improve the maize quality.