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木瓜多聚原花青素降解产物的液相色谱-电喷雾质谱分析 被引量:4

LC-ESI-MS Analysis of Degradative Products of Quince Polymeric Proanthocyanidins
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摘要 [目的]分析以茶多酚为亲核剂的木瓜原花青素降解反应产物。[方法]采用液相色谱-电喷雾质谱联用技术(LC-ESI-MS),分析主要降解反应产物。[结果]3个新产物的结构分别推测为(表)儿茶素-(4-8)-(表)没食子儿茶素、(表)儿茶素-(4-8)-(表)没食子儿茶素3-O-没食子酸酯和(表)儿茶素-(4-8)-(表)儿茶素3-O-没食子酸酯。[结论]该研究为木瓜原花青素降解产物进一步在医药品、保健食品、食品添加剂和化妆品等领域上的应用奠定基础。 [Objective] The study aimed to analyze the degradative products that was from the degradative reaction of quince polymeric proanthocyanidins with tea polyphenols as a nucleophile.[Method] LC-ESI-MS technique was adopted to analyze the structures of the main degradative products.[Result] Three new products were deduced as(epi)catechin-(4-8)-(epi)gallocatechin,(epi)catechin-(4-8)-(epi)gallocatechin 3-O-gallate,and(epi)catechin-(4-8)-(epi)catechin 3-O-gallate.[Conclusion] The results could provide scientific basis and support for the further application of the new type antioxidant oligomeric proanthocyanidins in the fields of pharmaceuticals,health foods,food supplement and additives,and costimetics,etc.
出处 《安徽农业科学》 CAS 2012年第31期15095-15096,15099,共3页 Journal of Anhui Agricultural Sciences
基金 云南省教育厅科学研究基金重大专项(ZD2010006)
关键词 木瓜多聚原花青素 降解产物 LC-ESI-MS Quince polymeric proanthocyanidins Degradative products LC-ESI-MS
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参考文献19

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二级参考文献121

共引文献83

同被引文献47

  • 1孙芸,徐宝才,谷文英,熊晓辉.葡萄籽原花青素的聚合度与抗氧化活性关系[J].食品与发酵工业,2006,32(10):41-46. 被引量:39
  • 2COS P, DE BRUYNE T, HERMANS N, et al. Proanthocyanidins in health care:Current and new trends [ J ]. Current Medicinal Chemistry, 2004,11 (10) : 1345-1359.
  • 3LOTITO S B, ACTIS-GORETYA L, RENART M L, et al. Influence of oligomer chain length on the antioxidant activity of procyanidins[ J ]. Bio- chemical and Biophysical Research Communications,2000,276 ( 3 ) :945-951.
  • 4ERLEJMAN A G, VERSTRAETEN S V, FRAGA C G, et al. The interaction of flavonoids with membranes:Potential determinant of flavonoid antioxidant effects[ J ]. Free Radical Research ,2004,38 ( 12 ) : 1311-1320.
  • 5HEMINGWAY R W. Reactions at the interflavonoid bond of proanthocyanidins[ M ]//HEMINGWAY R W, KARCHESY J. Chemistry and Sig- nificance of Condensed Tannins. New York : Plenum Press, 1989:265.
  • 6TANAKA T, YOSHITAKE N, ZHAO Ping, et al. Production of oligomeric proanthocyanidins by fragmentation of polymers [ J ]. Japanese Journal of Food Chemistry, 2007,14 ( 3 ) : 134-139.
  • 7TORRES J L, LOZANO C, JULIA L, et al. Cysteinyl-flavan-3-ol conjugates from grape pmcyanidins:Antioxidant and antiproliferative properties [ J ]. Bioorganic and Medicinal Chemistry, 2002,10 ( 8 ) : 2497-2509.
  • 8SELGA A, TORRES J L. Efficient preparation of catechin thio conjugates by one step extraction/depolymerization of pine ( Pinus pinaster) bark procyanidins [ J ]. Journal of Agricultural and Food Chemistry,2005,53 ( 20 ) : 7760-7765.
  • 9NONAKA G I, SUN B X, YUAN L,et al. Sulfur-containing proanthocyanidin oligomer composition and process for producing the same:WO, PCT WO 2004/103988 A1 [ P] ,2004.
  • 10MITJANS M, Del CAMPO J, ABAJO C, et al. Immunomodulatory activity of a new family of antioxidants obtained from grape polyphenols [ J ]. Journal of Agricultural and Food Chemistry,2004,52 (24) :7297-7299.

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