瘤胃体外连续培养系统的研制及模拟试验

Preparation of Continuous Culture System of Rumen in Vitro and Simulation test

[目的]研究饲料营养物质在瘤胃内发酵等动态变化规律,设计1套瘤胃体外连续培养系统。[方法]以活体为对照,对体外培养系统模拟瘤胃的发酵效果进行评价。[结果]模拟瘤胃发酵试验需2～3 d的适应期,3～9 d发酵状态相对稳定,效果较好,并且与活体瘤胃指标值呈一定规律性变化。[结论]瘤胃体外连续培养系统可作为研究活体瘤胃发酵的理想模型。

[Objective] The paper was to study the dynamic changes of forage nutrient substance fermentation in rumen,and a set of continuous culture system of rumen in vitro was designed.[Method]With in vivo as control,the simulating rumen fermentation effect in vitro culture system was evaluated.[Result]The simulation rumen fermentation test needed adaptive phase of 2-3 d,and the fermentation state was relatively stable within 3-9 d,with good effects.The test showed certain regularity variation with index value of rumen in vivo.[Conclusion]The continuous culture system of rumen in vitro could be used as the ideal model to study the rumen fermentation in vivo.