利用绒山羊原始生殖细胞培养胚胎干细胞的研究

Study on Embryonic Stem Cell from Cultured Primordial Germ Cells of Down Producing Goat

[目的]建立绒山羊胚胎干细胞体系。[方法]采用与其胎儿成纤维细胞共培养的方式从20 mm绒山羊胎儿生殖嵴及其周围组织分离克隆出大量原始生殖细胞(PGCS)集落。[结果]这些集落的细胞经过多次传代具有胚胎干细胞(ES)的诸多特征,如鸟巢状结构、AKP染色阳性、具有连续传代能力等。[结论]该细胞具有多能性,类似ES细胞。

[Objective] To establish embryonic stem cell system of goats.[Method] Numerous primordial germ cells colonies were derived from gonadal ridge and the surrounding tissues 20 millimeter fetuses of down producing goat.Primordial germ cells and goats embryonic fibroblasts obtained from a conceptus of equivalent gestational age were co-cultured.[Result] The colonies had some characteristics of embryonic stem cells,such as the morphology of nest-like,they continued to be AKP positive and the ability to be continuously passed.[Conclusion] These cells were pluripotent and ES-like cells.