苯酚降解菌SW34的鉴定

Identification and Characteristics of A Phenol-degrading Bacterium SW34

[目的]鉴定苯酚降解菌SW34。[方法]通过分析苯酚降解菌SW34的形态、生理生化性状及16S rRNA基因序列,鉴定该菌的种类。[结果]从焦化厂污水处理曝气池泥样中分离出具有降解苯酚能力的SW34菌株,根据其形态、生理生化性状初步鉴定属于短杆菌科(Brevibacteriaceae),其16S rRNA基因序列(在GenBank中的登录号为GU576981)与表皮短杆菌同源性高达99%以上。结合形态、生理生化特性以及16S rDNA序列分析,鉴定SW34菌株为表皮短杆菌(Brevibacterium lowffii),具有降解苯酚特性的表皮短杆菌此前未见报道。该菌株能够降解3.0 g/L浓度的苯酚,是处理高浓度苯酚废水的良好菌种资源。[结论]该研究为高浓度苯酚废水的处理提供了新的方法。

[Objective] The study aimed to identify phenol-degrading bacterium SW34.[Method] The bacteria species was identified through analyzing morphology,physiological and biochemical traits of degrading bacteria SW34 and 16S rRNA gene sequence.[Result] A bacterial strain capable of degrading phenol,designated SW34,was isolated from activated sludge of wastewater treatment plant of a coking chemical factory.Strain SW34 was identified as Brevibacteriaceae according to its morphological,physiological and biochemical properties,and the analysis of its 16S rRNA gene sequence that showed more than 99% similarity to several strains of Brevibacterium epidermidis.A strain of B.epidermidis able to degrade phenol had never been reported.Phenol could be decomposed effectively by strain SW34 when its concentration was lower than 3.0 g/L.It is a valid strain used for the treatment of high concentration of phenol wastewater.[Conclusion] The study could provide a new method for treatment of high concentration phenol wastewater.