DryLab软件辅助面制品中着色剂测定的快速高效液相色谱法的建立

With the Help of DryLab Software to Establish a Fast Method for the Determination of Colorants in Flour Products by HPLC

[目的]借助DryLab软件辅助建立面制品中8种着色剂的高效液相色谱分离二极管阵列检测器检测方法。[方法]选择面制品中8种着色剂作为分离研究对象,应用DryLab软件辅助建立合适的液相色谱分离方法,并将DryLab软件模拟运行的结果与实际分离结果进行了对照。[结果]外标法定量,各种着色剂的响应与其浓度在线性范围内均呈良好的线性关系,相关系数均0.999 9以上。在饼干中的回收率在90%以上,相对标准偏差(n=3)均在1以下,证明该方法的精密度和准确度良好。通过软件得到最佳分离条件,用Drylab软件进一步模拟出相应的色谱图,最后用真实的试验结果加以验证,大幅缩短了色谱方法的开发周期。[结论]该方法前处理大为简化,各种成分在色谱柱上分离度较好,试验平行性较佳,回收率较好,检测速度快,可对面制品中多种着色剂同时测定。

[Objective] With the help of DryLab software to establish a method for the determination of 8 colorants in flour products by High Performance Liquid Chromatographic with PDA detection.[Method] Samples were ultrasonically extracted centrifugalize and filtered,then the sample solutions were injected into HPLC with PDA detection.[Result] The response of each analyte showed a good linearity with a correlation coefficient not less than 0.999 9.The recoveries for biscuits were more than 90%,with the relative standard deviations（n=3） of less than 1.0,which demonstrated the good precision and accuracy of the present method.The method is rapid,accurate,reproducible.At the mean time,the optimal chromatogram was simulated and presented by Drylab,which was almost the same with actual chromatogram,greatly shorten the development cycle of chromatographic method.[Conclusion] The method has many advantages,such as simplified pretreatment,rapid detection speed,good recovery rate,et al.