偏侧帕金森大鼠模型各个发病时期多巴胺能系统的动态监测

The unilateral 6-hydroxydopamine lesion rats' dynamic supervision about the nigrostriatal dopaminergic system in various stages of its pathogenesis

目的动态监测帕金森大鼠不同发病阶段多巴胺能系统的改变,掌握其变化规律和各指标间的量化关系,为进一步实验提供动物治疗模型。方法偏侧两点注射法制作帕金森病大鼠模型,模型成功两周后给予小剂量左旋多巴治疗一周。每周检测纹状体多巴胺(DA)含量,黑质酪氨酸羟化酶(TH)染色和尼氏染色,观察行为学改变。结果一周后即发病的代偿期,行为学开始出现向健侧的旋转;两周后即失代偿期,旋转次数加重至最高峰。前两周多巴胺含量分别较正常对照侧减少16.7%和80%,TH阳性细胞分别下降47.97%和93.28%,尼氏细胞下降31.4%和46.4%.给予多巴胺治疗一周后,旋转圈数及TH染色、尼氏染色没有显著性改变,多巴胺含量明显增加到58.3%。结论短期小剂量给予左旋多巴治疗晚期PD仅能提高多巴胺含量。

Objective To dynamically supervise the changes of the nigrostriatal dopaminergic system of rats with unilateral 6-hydroxydopamine（6-OHDA） lesion in various stages of its pathogenesis and to find out the rules of the changes,and to build up a model of treatment for the further research.Methods The unilateral lesioned rat models of Parkinson＇s Disease with 6-OHDA were treated with levodopa for 14 days after the successful models were made.Each 7 days during the course,we measure the behavioral rotations,count the numbers of the tyrosine hydroxylase（TH）positive cells,the dopamine（DA） contents,and make the Nissle stain.Results At the first and the second time points,the concentration of DA collapsed by 16.7% and 80%,the numbers of TH positive cells decreased by 47.97% and 93.28%,besides the Nissl＇s bodies were cut by 31.4% and 46.4%.After the treatment with low dose of levodopa,the rotational behaviour and the number of the TH positive cells and the Nissl＇s bodies didn＇t change obviously.Only the concentration of DA increased to 58.3% of that of the control group.Conclusion These items all positively related to each other during the pathogenesis.The low-dose administration of levodopa for a short time only had some effect on the ascending of the concentration of dopamine for in advanced Parkinson＇s disease.