乙型肝炎和丙型肝炎患者血清抗肝特异性抗体测定

Expression of the serum liver specific antibodies with HBV and HCV infected

目的:分析乙型肝炎病毒(hepantitis B virus,HBV)和丙型肝炎病毒(hepantitis C virus,HCV)感染患者血清中肝特异性抗体水平,探讨其与肝细胞损伤的关系。方法:收集2011年1月至2011年10月川北医学院附属医院HBV和HCV感染患者和健康体检者血清,检测患者丙氨酸氨基转移酶(alannine aminotransferase,ALT)和总胆红素水平;通过酶联免疫吸附法检测抗肝细胞膜特异性脂蛋白(liver specific lipoprotein,LSP)和抗去唾液酸糖蛋白受体(asialoglycoprotin receptor,ASGPR)的血清水平;以ALT>40分为肝功能异常组与正常组。使用SPSS13.0统计软件分析结果,P<0.05认为有统计学意义。结果:HBV(H)与健康对照组比较anti-LSP、anti-ASGPR具有统计学差异,HBV组患者anti-ASGPR水平与ALT具有相关性。HCV(H)与健康对照组比较anti-LSP具有统计学差异,HCV组患者anti-LSP与anti-ASGPR具有相关性。HBV患者anti-LSP、anti-ASGPR表达水平较HCV患者高。结论:HBV、HCV感染后,anti-LSP和anti-ASGPR表达高于健康对照组,ALT水平升高时抗肝特异性抗体表达明显增高,表明其可能作为监测肝细胞损伤的特异性免疫学指标。

Objective ：To analyze the liver specific antibodies expression in serum who with HBV and HCV infected and approach its role. Methods：The samples were collected in Affiliated Hospital of North SiChuan Medical College from January 2011 to October 2011, who with HBV and HCV infected and the healthy check-up as controls, and the ALT and TBIL level be measured at the same time, by ELIAS kit to test anti-LSP and anti-ASGPR expressing. Use SPSS13.0 analysis the results, P 〈 0.05 be viewed as statistically signifi- cance. Results：The level of anti-LSP, anti-ASGPR was significance difference between HBV （H） and health control group and there was correlation between anti-ASGPR and ALT in HBV groups. The level of anti-LSP was significance difference between HCV（H） and health control group and there was correlation between anti-LSP and anti-ASGPR in HCV groups. The level of anti-LSP and anti-ASGPR in the groups of HBV expressed more than HCV groups. Conclusions：The level of the anti-LSP and anti-ASGPR was higher in in who with HBV and HCV infected, so they may be help to monitor the injury in hepatocytes with hepatitis virus infected.