不同分期股骨头坏死组织TGF-β的表达

Expression of transforming growth factor-β in patients with femoral head necrosis of various stages

目的:研究股骨头坏死不同时期股骨头组织内的转化生长因子β(TGF-β)的表达情况。方法:取20()9年11到2011年12月惠州市第三人民医院收治的股骨头坏死患者股骨头组织,每期(0、Ⅰ、Ⅱ、Ⅲ、Ⅳ期)各10份标本。采用ELISA法检测组织匀浆中TGF-β的表达情况。结果:股骨头坏死Ⅱ期、Ⅰ期和0期TGF-β水平[(54.76±9.13)、(96.89±6.52)、(125.86±9.45)pg/mL]较Ⅳ期和Ⅲ期TGF-β水平[(12.76±1.39)、(31.34±10.48)pg/mL]差异有统计学意义(P<0.05),Ⅰ期和0期TGF-β水平较Ⅱ期TGF-β水平差异有统计学意义(P<0.05)。结论:股骨头坏死不同分期的TGF-β的表达不同,随着分期的增加TGF-β的表达量逐渐减少。

Objective：To investigate the expression of transforming growth factor-β in patients with ischemic necrosis of femoral head （ INFH ） at various stages. Methods： We collected the specimens of femoral head in patients with INFH at stages 0 （ n = 10）, Ⅰ （ n = 10）,Ⅰ （ n = 10）, m （ n = 10） and IV （ n = 10） from the first department of orthopedics ,The Third People＇s Hospital of Huizhou between November 2009 and December 2011. The expression of transforming growth factor-β in the tissue homogenate was detected by using enzyme-linked immunosorbent assay. Results ： Patients with INFH at both stage Ⅲ and stage Ⅳ [ （ 12.76 ± 1.39 ） pg/mL and （ 31.34 ± 10.48 ） pg/mL] yielded considerably lower levels of TGF-β （ all P 〈 0.05 ） as compared with those at stages 0, 1 and Ⅱ [ （ 54.76 ± 9. 13 ） pg/mL, （ 96.89 ± 6.52 ） pg/mL and （ 125. 86 ± 9.45 ） pg/mL ]. Furthermore, the level of TGF-β at stages 0 and I differ statistically from that at stage Ⅱ （ P 〈 0. 05 ）. Conclusion：Expression of TGF-β may vary at different stages of INFH and gradually decreases with the advancement of staging.