维生素D_3联合地塞米松诱导大鼠破骨细胞的体外培养

Induction of the Formation of Rat Osteoclast with the Combination of Vitamin D_3 and Dexamethasone in Vitro

目的:观察VitD3联合地塞米松对大鼠骨髓体外培养破骨细胞的影响,探索提高破骨细胞体外培养效果的有效途径。方法:实验分为两组,以维生素D3骨髓诱导法为对照组,以维生素D3联合地塞米松诱导SD大鼠骨髓细胞为实验组,通过Trap染色、甲苯胺蓝染色和扫描电镜观察鉴定破骨细胞,并对计数结果进行统计学分析。结果:实验组与对照组均获得Trap染色阳性及在体外有噬骨能力的破骨细胞,实验组破骨细胞计数(培养第7天)多于对照组,有统计学差异(P<0.05)。结论:与维生素D3单独诱导相比,维生素D3联合地塞米松体外诱导大鼠破骨细胞的效率更高。

Objective： To establish a method for inducing rat bone marrow cells into osteoclasts in vitro, and to explore the effective way to improve the effect of culturing osteoclasts in vitro. Methods： Vitamin D3 bone marrow cell induction method served as control. Vitamin D3 combined with dexam- ethasone were used to induce Sprague-Dawley rat bone marrow cells into osteoclasts. The osteoclasts were identified by Trap staining, toluidine blue staining and scanning electron microscope （SEM） ob- servation. The count results of osteoclasts were analyzed statistically. Results： Trap - positive osteo- clasts which had the ability of bone absorption were obtained in the two groups. Statistical differences were found in the amounts of osteoclasts between the two groups （P 〈 0. 05 ）. Conclusion： Compared with inducing by vitamin D3 alone, inducing the formation of rat osteoclasts by vitamin D3 combined with dexamethasone have better effect.