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维生素D_3联合地塞米松诱导大鼠破骨细胞的体外培养 被引量:2

Induction of the Formation of Rat Osteoclast with the Combination of Vitamin D_3 and Dexamethasone in Vitro
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摘要 目的:观察VitD3联合地塞米松对大鼠骨髓体外培养破骨细胞的影响,探索提高破骨细胞体外培养效果的有效途径。方法:实验分为两组,以维生素D3骨髓诱导法为对照组,以维生素D3联合地塞米松诱导SD大鼠骨髓细胞为实验组,通过Trap染色、甲苯胺蓝染色和扫描电镜观察鉴定破骨细胞,并对计数结果进行统计学分析。结果:实验组与对照组均获得Trap染色阳性及在体外有噬骨能力的破骨细胞,实验组破骨细胞计数(培养第7天)多于对照组,有统计学差异(P<0.05)。结论:与维生素D3单独诱导相比,维生素D3联合地塞米松体外诱导大鼠破骨细胞的效率更高。 Objective: To establish a method for inducing rat bone marrow cells into osteoclasts in vitro, and to explore the effective way to improve the effect of culturing osteoclasts in vitro. Methods: Vitamin D3 bone marrow cell induction method served as control. Vitamin D3 combined with dexam- ethasone were used to induce Sprague-Dawley rat bone marrow cells into osteoclasts. The osteoclasts were identified by Trap staining, toluidine blue staining and scanning electron microscope (SEM) ob- servation. The count results of osteoclasts were analyzed statistically. Results: Trap - positive osteo- clasts which had the ability of bone absorption were obtained in the two groups. Statistical differences were found in the amounts of osteoclasts between the two groups (P 〈 0. 05 ). Conclusion: Compared with inducing by vitamin D3 alone, inducing the formation of rat osteoclasts by vitamin D3 combined with dexamethasone have better effect.
出处 《贵阳医学院学报》 CAS 2012年第5期486-489,共4页 Journal of Guiyang Medical College
基金 贵州省优秀科技教育人才省长专项基金(黔省专合字2007-117号) 贵州省科学技术基金【黔科合J字(2009)2308号】
关键词 破骨细胞 细胞 培养的 维生素D3 地塞米松 osteoclasts cell, cultured vitamin D3 dexamethasone
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