泰山地区回民糖耐量异常群体VDR受体基因多态性分析

Analysis of VDR gene polymorphism of Hui ethnic group with impaired glucose tolerance in Taishan region

目的探讨泰山地区回民群体糖耐量异常与维生素D受体基因多态性相关性。方法采用聚合酶链反应-限制性长度多态性技术,对回民糖耐量异常者和糖耐量正常回民检测VDR基因(Apa-I和Fok-I)多态性及等位基因分布频率,并检测VDR蛋白血液含量。结果糖耐量异常组Apal-I多态性AA、Aa和aa基因型分布率为10%、34.6%和55.4%,正常对照组相应为13.8%、42.5%和43.7%:糖耐量异常组Fok-I酶切多态性FF、Ff和ff基因型分布率为23.2%、41.1%和35.7%,正常对照组相应为13.8%、42.5%和43.7%;结论泰山地区回民群体糖耐量异常与维生素D受体基因多态性相关;VDR蛋白血液浓度和胰岛素抵抗在各型间显示差异性。

Objective： To investigate the vitamin D receptor gene polymorphism of Hui ethnic group with impaired glucose tolerance in Taishan region.Methods： The VDR gene（Fok,-I and Apa-I） polymorphism and allele distribution frequency were detected with polymerase chain reaction-restriction fragment length polymorphism（RFLP） method on sante Hui ethnic group with impaired glucose tolerance and normal Hui ethnic group,and also VDR protein in the blood of each group were detected.Results： In the impaired glucose tolerance group,VDR gene（Apal-I） polymorphism included AA（10%）,Ff（34.4%） and ff（55.6%） three genotype,while they were 13%（AA）,42.5%（Aa） and 43.7%（aa） in the normal control group;VDR gene（Fok-I） polymorphism included FF,Ff and ff genetype,the gene rate was 23.4（AA）,41.1%（Aa） and 35.3%（aa） in impaired glucose tolerance group and 13.8%（AA）,42.5%（Aa） and 43.7%（aa） in the normal control group.Conclusion： Hui ethnic group in Taishan region with impaired glucose tolerance is related to the vitamin D receptor gene polymorphism and VDR protein concentration in peripheral blood and insulin resistance is different significiantly among each genetype.