阿维A对寻常性银屑病患者外周血MCP-1及MIP-1α表达水平的影响

Effect of Acitretin on MCP-1 and MIP-1α of the Peripheral Blood in Patients with Psoriasis Vulgaris

目的探讨阿维A治疗寻常性银屑病(PV)的疗效以及对PV患者血清单核细胞趋化蛋白-1(MCP-1)及巨噬细胞炎性蛋白-1α(MIP-1α)的影响,进一步阐明阿维A治疗PV的作用机制。方法 38例中、重度PV患者口服阿维A治疗8周,以银屑病皮损面积和严重程度(PASI)评分评价疗效;采用双抗体夹心法(ELISA)检测正常对照组以及PV患者阿维A治疗前后血清MCP-1及MIP-1α的表达水平,并与40例正常对照组比较。结果阿维A治疗中、重度PV疗效显著,治疗后PASI评分明显下降(P<0.01)。PV患者外周血MCP-1及MIP-1α表达水平分别为(267.95±16.87)pg/mL,(1319.47±165.25)pg/mL,明显高于正常对照组(87.36±9.63)pg/mL,(479.24±35.71)pg/mL(P<0.01);阿维A治疗后血清MCP-1(91.71±11.25)pg/mL,与治疗前相比明显降低(P<0.01),同正常对照组比较差异无统计学意义(P>0.05);治疗后血清MIP-1α(956.58±87.39)pg/mL,较治疗前显著下降(P<0.01),仍显著高于正常对照组(P<0.01)。结论阿维A治疗中、重度银屑病疗效明显,可能通过调节外周血MCP-1及MIP-1α表达水平来发挥治疗银屑病的作用。

Objective To investigate the therapeutic effect of acitretin on psoriasis vulgaris （PV） and study the effect of acitretin on serum levels of monocyte chemoattractant Protein-1 （ MCP-1 ） and macrophage inflammatory pro- tein-1α （ MIP-1α）, further clarify the mechanism of Acitretin. Methods Thirty-eight patients with moderate to severe PV were treated with acitretin for 8 weeks. The efficacy of treatment was evaluated by Psoriasis Ar- ea and Severity Index （PASI） scores. Enzyme-linked immunosorbent assay （ELISA） was used to detect the serum levels of MCP-1 and MIP-1α in 40 healthy controls and 38 patients with PV before and after treat- ment. Results The mean PASI scores were significantly decreased after treatment with acitretin （P 〈 0.01 ）. Serum levels of MCP-1 and MIP-1α were（267.95 ± 16.87） pg/mL and（ 1 319.47 ± 165.25 ） pg/mL respectively. They were significantly higher in PV patients than those in healthy controls （87.36 ± 9.63 ） pg/mL and （ 479.24 ± 35.71 ） pg/mL respectivdy （ P 〈 0.01 ）. After treatment with acitretin, serum level of MCP-1 （91.71 ± 11.25） pg/mL was significantly decreased than that before treatment （P 〈0.01 ）, and had no significant difference with that in healthy controls （P 〉 0.05 ） ; Serum level of MIP-1α （956.58 ± 87.39） pg/mL was significantly decreased than that before treatment （P 〈 0.01 ）, but it was still higher than that in healthy controls （P 〈 0.01 ）. Conclusion Acitretin is significantly effective in the treatment of moderate to severe PV. It may affect serum levels of MCP-1 and MIP-1α to play a role in the treatment of psoriasis.