大连、沈阳两地须癣毛癣菌复合体的分子鉴定及DNA分型

Study on Molecular Identification and DNA Typing of Trichophyton Mentagrophyes Complex in Dalian and Shenyang

目的探讨大连和沈阳两地须癣毛癣菌复合体的主要组成菌种及主要组成菌种肉芽肿株和体癣株DNA分型有无差异。方法选取大连和沈阳地区已行表型鉴定的须癣毛癣菌16株(12株体癣株,4株肉芽肿株),通过PCR扩增核糖体DNA(rDNA)的内转录间隔区(ITS),测序后利用数据库进行序列比对,对须癣毛癣菌复合体进行鉴定;PCR扩增rDNA非转录间隔区(NTS)的3个串联重复亚单位S0,S1和S2区,进行种内分型,比较菌株间型别的差异性。结果大连、沈阳两地的16株须癣毛癣菌中,2株鉴定为断发毛癣菌,1株鉴定为苯海姆节皮菌,13株鉴定为万博节皮菌;3对不同引物扩增13株万博节皮菌NTS区,共产生8种特征性带型;万博节皮菌的体癣株和肉芽肿株的带型除S2区外,在S0,S1区及总区间均有差异。结论大连、沈阳两地须癣毛癣菌复合体的主要组成菌种为万博节皮菌;万博节皮菌肉芽肿株和体癣株NTS区的带型有差异。

Objective To investigate the main constituent stains of T. mentagrophyes complex and whether the differ- ences exist between the granuloma and tinea strains of Trichophyton mentagrophyes （ T. mentagrophyes）. Methods Sixteen strains of T. mentagrophyes （i. e. 12 strains from tinea and 4 from granuloma strains） from Dalian and Shenyang were chosen to take phenotype identification. The rRNA gene internal transcribed spacer regions（ITS） of these strains were amplified by PCR,then the database was used to take sequence a- lignment in order to identify T. mentagrophyes complex. Three novel tandem repeat subelements （TRSs） of the rDNA nontranscribed spacer regions （NTS）, S0, S1 and S2, were also amplified by PCR, then intraspecif- ic typing was taken in order to observe the differences among these strains. Results Among these 16 strains of T. mentagrophyes,two strains were identified as T. tonsurans,one strain was identified as A. benhamiac, and the other strains were identified as T. interdigitale. 13 strains of T. interdigitale which were amplified by three different polymorphic loci totally produced 8 kinds of strain-characteristic banding patterns. There were some differences in SO, S1 and the full NTS regions of granuloma and tinea strains of T. interdigitale, except for S2 region. Conclusion T. interdigitale was the main constituent strain of T. mentagrophyes complex in Dalian and Shenyang. There are some differences in NTS regions between the granuloma and tinea strains of T. mentagrophyte.