慢性心肌缺血小型猪的氧化应激反应指标观测

Observation on Oxidative Stress in Chronic Myocardial Ischemia in Mini-pig

目的观测慢性心肌缺血小型猪的氧化应激反应指标。方法15只巴马小型猪随机分成3组，即正常对照（ctr）组，高脂（HF）组和慢性心肌缺血（CMI）组，每组5只。采用维生素D3和异丙肾上腺素复合高脂饮食建立慢性心肌缺血模型，连续24周。造模24周后，观察各组血清cK—MB、血小板聚集率及心肌组织中SOD、MDA、GSH—Px、N0、LD和ATP酶含量的变化。结果与Ctr组比，CMI组血清CK-MB活性、PAR、心肌组织中MDA和LD含量均显著升高（P〈0．05，P〈0．01），心肌组织中SoD、SoD／MDA比值、GSH—Px、N0、Na＋－K＋－ATP、Ca＋＋-ATP和Mg＋＋-ATP酶活性均明显降低（P〈0．05，P〈0．01），而HF组PAR和心肌组织中sOD／MDA比值、MDA含量均升高显著（P〈0．05，P〈0．01），心肌组织中soD、GsH—Px、Na＋-K＋-ATP、Mg＋＋-ATP酶活性均降低显著（P〈0．05）；与HF组比，CMI组血清CK—MB活性明显高于HF组（P〈0．01），心肌组织中Na＋-K＋-ATP酶和Ca＋＋-ATP活性显著低于HF组（P〈0．05，P〈0．01）；相关分析显示，CMI组cK-MB活性与PAR和MDA含量呈显著正相关（P〈0．05，P〈0．01），与soD、sOD／MDA、GSH．Px、NO呈显著负相关（P〈0．05，P〈0．01）；血小板聚集率与cK-MB和MDA呈显著正相关（P〈0．05），与sOD、s0D／MDA和Na＋-K＋-ATP酶活性呈显著负相关（P〈0．05，P〈0．01）；心肌组织中LD含量与MDA呈显著正相关（P〈0．05），与SOD／MDA、Na＋-K＋-ATP、Ca＋＋-ATP和Mg＋＋-ATP呈显著负相关俨〈0．05）；心肌组织中NO水平与SoD、SOD／MDA、GSH—Px呈显著正相关（P〈0．05，P〈0．01），与CK—MB和MDA呈显著负相关（P〈0．05）。

Objective To observe the effects of oxidative stress on chronic myocardial ischemia in mini-pigs. Methods Fifty-five Bama mini-pigs were randomly divided into three groups： normal control （Ctr） group, high-fat （HF） group and chronic myocardial ischemia （CMI） group, five animals in each group. The chronic myocardial ischemia model was made induced by vitamin D3, isoproterenol and high-fat diet for 24 weeks. The changes of phosphokinase isoenzyme （CK-MB）, platelet aggregation rate （PAR） and the SOD, MDA, GSH-Px, NO, LD, ATPase in myocardial tissues were detected. Results Compared with normal control group, the serum CK-MB activity, PAR, the myocardial tissues of MDA and LD contents in CMI group were significant increased （P〈0.05, P〈0.01）, the myocardial tissues of SOD, SOD/MDA ratio, GSH-Px, NO, Na＋-K＋-ATPase, Ca＋＋-ATPase and Mg＋＋-ATPase in CMI group were lowered （P〈0.05, P〈0.01）. PAR and the myocardial tissues of SOD/MDA ratio and MDA content in HF group were significant increased （P〈0.05, P〈0.01）, while the myocardial tissues of SOD, GSH-Px, Na＋- K＋-ATPase and Mg＋＋-ATPase were significant decreased （P〈0.05）. Compared with HF group, the serum CK-MB activity in CMI group was significant higher than that of HF group （P〈0.01） and the myocardial tissues of Na＋-K＋-ATPase and Ca＋＋-ATPase were significant lower than those of HF group （P〈0.05, P〈0.01）. Correlation analysis showed that there was a positive correlation between CK-MB and PAR, MDA in CMI group （P〈0.05, P〈0.01）, and a significant negative correlation between CK-MB and SOD, SOD/MDA, GSH-Px, NO respectively（P〈0.05, P〈0.01）. The platelet aggregation rate was positively correlated with CK-MB and MDA （P〈0.05）, and was negative relation with SOD, SOD/MDA and Na＋- K＋-ATPase （P〈0.05, P〈0.01）. There was a significant positively correlation between LD and MDA in myocardial tissues （P〈0.05）, and LD was negatively correlated with SOD/MDA, Na＋-K＋-ATPase, Ca＋＋-ATPase and Mg＋＋-ATPase （P〈0.05）. NO level was positively correlated with SOD, SOD/MDA, GSH-Px in myocardial tissues（P〈0.05, P〈0.01）, and was negatively related with CK-MB and MDA （P〈O.05）. Conclusion Oxidative stress injury was participated in the formation of chronic myocardial ischemia.