摘要
目的通过观察糖尿病大鼠肾组织血管紧张素转换酶2（ACE2）的表达变化，探讨番茄红素的肾保护作用机制。方法实验设5组：正常对照组（NC组）、糖尿病模型对照组（DM组）、番茄红素高剂量组（20mg·mgnd-i）、中剂量组（10mg·mg-1·d-1）和低剂量组（5mg·mg-1·d-1）。8周后，测定大鼠血液和尿液相关指标，免疫组织化学和Westernblotting法检测肾组织ACE2蛋白的表达。结果DM组和番茄红素治疗组的血糖、胰岛素、血肌酐、尿素氮、血管紧张素Ⅱ及24h尿蛋白均显著高于NC组（P〈0．05）；经番茄红素治疗后，血肌酐、尿素氮、血管紧张素Ⅱ和24h尿蛋白均显著改善（P〈0．05）；与NC组相比，DM组的ACE2阳性表达和蛋白表达水平均显著降低（P〈0．01），而番茄红素高、中剂量组的ACE2阳性表达和蛋白表达水平则均显著高于DM组（P〈0．05），其中高剂量效果更为显著（P〈0．01）。结论番茄红素能降低糖尿病肾病（DN）大鼠尿蛋白、改善肾功能及提高肾组织ACE2蛋白的表达。
Objective To observe the expression changes of angiotensin-converting enzyme 2 （ACE2） in kidney tissue of rats with diabetic nephropathy, and explore the renal protection mechanism of lycopene. Methods The rats were divided into 5 groups： nomal control group, diabetic nephropathy （DM） model group, and Lycopene treated groups with high-dose （20 mg·kg-1·d-1）, middledose （10 mg·kg-1·d-1）, and low-dose （5 mg·kg-1·d-1） respectively. After 8 weeks, the blood and urine index was measured. The expression level of ACE2 protein in kidney tissue was detected respectively by immunohis- tochemistry and western blotting. Results The concentrations of blood glucose, serum insulin, creatinine, urea nitrogen, AnglI and urine protein in lycopene and diabetes groups were all significantly higher than those of normal control group （P〈0.05）; After treated by lycopene, serum creatinine, urea nitrogen, AnglI and urine protein were greatly improved （P〈0.05）. The expression of ACE2 protein in diabetes group was greatly decreased than that of normal control group （P〈0.01）, while ACE2 protein levels in the high- and middle-dose groups treated with lycopene were obviously up-regulated （P〈0.05）, especially in high-dose lycopene group （P〈0.01）. Conclusion Lycopene can decrease urine protein, improve the renal function, and up-regulate the expression of ACE2 protein in diabetic nephropathy.
出处
《实验动物与比较医学》
CAS
2012年第5期421-425,共5页
Laboratory Animal and Comparative Medicine
基金
浙江省实验动物科技计划项目（2009F80024）
