特发性血小板减少性紫癜患者Th1/Th2的细胞因子水平及临床意义

Detection and clinical significance of Th1/Th2 cytokines in patients with idiopathic thrombocytopenic purpura

目的:检测特发性血小板减少性紫癜(ITP)患者外周血Th1(IL-2、IFN-γ)及Th2(IL-4、IL-10)细胞因子的变化,探讨ITP患者发病与Th1/Th2优势活化状态之间的关系。方法:通过酶联免疫吸附试验(ELISA)法检测30例ITP患者治疗前、治疗后及26例健康志愿者外周血清中IL-2、IFN-γ、IL-4、IL-10水平。结果:ITP患者治疗前IFN-γ及IL-2因子水平高于治疗后和对照组,差异有统计学意义(P<0.05),而IL-4及IL-10因子治疗前水平低于治疗后和对照组,差异有统计学意义(P<0.05)。结论:Th1型细胞因子介导的免疫应答在ITP的发病机制中占主导地位,糖皮质激素治疗可调整Th1/Th2细胞因子的偏移状态。

AIM： To observe Thl （ IL-2 and IFN-y） and Th2 （IL-4, IL-10 ） cytokine changes in patients with idiopathic thrombocytopenic purpura （ITP） and explore the correlation between ITP development and Th1/Th2. METHODS： A total of 30 patients with ITP and 26 healthy volunteers were enrolled in this study. Serum levels of IL-2, IFN-y, IL-4, and IL-10 were detected by enzyme-linked im- munosorbent assay （ELISA） before and after treatment. RESULTS： The serum levels of IFN-y and IL-2 in patients with ITP before the glucocorticoid treatment were significant- ly higher than those after the treatment and in the control groups （P〈0.05）, while the serum levels of IL-4 and IL-10 in patients with ITP before treatment were significantly lower than those after the treatment and in the control group （ P 〈 0.05）. CONCLUSION： Th1/Th2 imbalance plays a promi- nent role in the development of ITP, and glucocorticoid treatment helps to restore the Th1/Th2 in the patients with ITP.