IBA和6-BA浓度配比对诱导大豆下胚轴不定芽的影响

Effect of IBA and 6-BA Ratio on Adventitious Shoot Induction from Hypocotyl in Soybean

以大豆黑农44下胚轴为外植体,研究不定芽诱导培养基中IBA和6-BA浓度及配比对不定芽诱导与伸长的影响。结果表明,以MS基本培养基+1.0 mg·L-16-BA为大豆无菌苗培养基,在诱导培养基中不添加6-BA时有利于下胚轴不定芽的诱导,添加0.1 mg·L-1IBA时,培养14 d不定芽诱导率可达96.69%,每个下胚轴形成2.31个芽(芽长≥0.5 cm);不定芽诱导培养基中添加高浓度6-BA和IBA组合不利于大豆下胚轴不定芽的诱导与伸长。

Hypocotyl from soybean Heinong 44 was used as the explants to investigate the effects of IBA and 6-BA and the concentration ratio on the adventitious shoot induction and elongation.The higher induction rate of adventitious shoots from hypocotyls was observed in the induction MS medium without 6-BA while the seeds germinated on MS medium＋1.0 mg·L-16-BA.Induction rate and number of adventitious shoots reached 96.69% and 2.31（shoot length≥0.5 cm）respectively cultured for 14 days on induction MS medium with addition of 0.1 mg·L-1IBA.Shoot induction medium with higher concentration of 6-BA and IBA significantly reduced the induction and elongation of adventitious shoots from hypocotyl of soybean.