大鼠MSCs的体外分离、培养及鉴定的实验研究

Experimental study on isolation,culture and identification of MSCs in rats

[目的]体外分离、培养及鉴定大鼠骨髓间充质干细胞(MSCs)。[方法]密度梯度离心法体外分离、培养7周龄SD大鼠MSCs,利用差速贴壁原理纯化MSCs,并通过形态学观察、细胞表面抗原标志物、多细胞系诱导分化对其进行鉴定。[结果]第3代(P3代)不表达抗原CD34,表达抗原CD44,符合MSCs表面标志物特征。[结论]密度梯度离心法能够建立稳定的MSCs体外分离培养体系。

[Objective] To isolate, culture and purify bone marrow mesenchymal slem cells（MSCs）. [Methods] MSCs from the rat bone marrow was obtained and purified in vitro by the method of gradient centritilgation combining with adherence cuhure. They were assess by immunoeytochemistry. [Results] The samples of MSCs of third passage couht express CD44, but not CD34, according to the cbaracleristics of the MSCs. [Conclusion] The method of density gradient centrifugation can establish a stable system to separate and culture MSCs in vitro.