RP-HPLC同时测定维吾尔药材无花果叶中芦丁和补骨脂素含量

RP-HPLC Determination of Rutin and Psoralen in Leaves of Ficus carica L.

目的:建立维吾尔药材无花果叶薄层色谱鉴别及HPLC含量测定方法。方法:采用薄层色谱法鉴别无花果叶;使用Kromasil C18柱(250mm×4.6mm,5μm),流动相:甲醇-0.4%磷酸(40∶60);流速:1.0mL·min-1;检测波长:245nm;柱温:25℃;同时测定无花果叶中芦丁和补骨脂素含量。结果:薄层色谱法鉴别色谱斑点清晰,专属性强;芦丁和补骨脂素含量测定线性范围分别为0.1024～1.024μg,r=0.9998,n=5;0.041～0.7175μg,r=0.9999,n=5。芦丁平均回收率为103.7%(RSD=0.89%,n=6),补骨脂素平均回收率为90.9%(RSD=1.67%,n=6)。结论:该方法简便、准确,专属性强,可用于无花果叶药材的质量控制。

Objective： To establish a HPLC method for determination of rutin and psoralen in Leaves of Ficus carica L.. Methods： Fig Leaves were identified by TLC ; The separation was performed on Kromasil Clscolumn （250 mm x4. 6 ram, 5 μm）, with methaonl-0. 4% phosphoric acid （40：60） as mobile phase, a flow rate of 1.0 mL rain-l The UV detection wavelength was 245 nm. The column temperature was 25 C. Results： The calibration curves of turin were linear in the ranges of 0. 102 4 - 1. 024 μg （ r = 0. 999 8, n = 5 ）. The calibration curves of psoralen were linear in the ranges of 0. 041 - 0. 717 5 μg（ r = 0. 999 9, n = 5 ） The average recoveries of rutin and psoralen were 103.7% and 90. 9%, respectively. Conclusion： The method is simple, accurate and sensitive. It can be used for quality control of fig leaves.