哺乳动物NMDA受体NR3亚基的比较基因组学研究

Comparative Genomics Analysis for NR3 Subunit of Mammal NMDA Receptor

目的:通过比较基因组学方法对哺乳动物NR3亚基进行分析,探讨其种间功能及结构差异,并预测其功能。方法:应用人类NR3A和NR3B所编码氨基酸序列作为检索探针,通过TBLASTN检索数据库,并应用生物软件进行进化分析及序列对比。结果:共检索到30个哺乳动物NR3A及NR3B基因,其中28个具有完整CDS。进化树显示相同种属均形成独立分支,与人类相似度越高的物种进化距离越近,氨基酸种间比较显示邻接半胱氨酸序列及loop1结构保守性较好,而富含脯氨酸序列在灵长类和啮齿类存在差异。结论:NR3A亚基在人类和啮齿类可能存在功能差异,应用动物模型进行实验应考虑差异可能带来的影响,此外,可推断哺乳动物间NR3亚基富含半胱氨酸序列及loop1结构产生的生理过程及功能相似。

Objective： To discuss the inter-species difference of rnamrnal NR3 subunit function and conformation and predict the NR3 subunit function with comparative genomics method. Method： The amino acids sequences coded by human NR3A and NR3B genes were used as the probes to search in the online database by TBLASTN. And the evolutionary analysis and sequence alignment were performed on biological sottware. Results： 30 mammal NR3 （NR3A and NR3B） sequences were acquired, including 28 items with complete CDS region, The animals belonged to the same species were grouped in the independent cluster in the evolution tree, and the species with the higher similarity to human also have the smaller evolutionary distance to human. The vicinal cysteine residues and loopl were conservative among the selected mammal species. However, the alignment difference of proline rich motif was observed between selected primate and rodents. Conclusion： The possible function difference between selected primate and rodents ought to be considered in the experiments using animal model. Besides, it could be inferred that the physiological process and function mediated and produced by vicinal cysteine residues and loop 1 were similar.