高糖环境对Mü ller细胞谷氨酸转运体及谷氨酰胺合成酶表达的影响

The Effect of High Glucose on the Expression of Glutamate Transporter and Glutamine Synthetase in Müller Cell

目的:研究高糖环境对原代培养新生7天SD乳鼠视网膜Mü ller细胞谷氨酸转运合成系统的影响及其可能机制。方法:新生7天SD乳鼠视网膜Mü ller细胞原代培养并模拟高糖环境构建乳鼠视网膜mü ller细胞体外高糖环境模型。处理分为3组:对照组,高糖组,高糖+白藜芦醇干预组。培养时间为24 h,通过western blot等检测方法,对照观察各组Müller细胞谷氨酸转运体(GLAST)、谷氨酰胺合成酶(GS)的表达情况。结果:模拟高糖环境可以造成新生SD乳鼠视网膜Müller细胞谷氨酸转运体(GLAST)表达的降低(0.225 fold VS control,P<0.05),并导致其表达的谷氨酰胺合成酶(GS)表达水平的显著降低(0.653 fold VScontrol,P<0.05);而干预药物白藜芦醇作用后可明显逆转新生SD乳鼠Mü ller细胞谷氨酸转运体(GLAST)(1.133 fold VS H Ggroup,P<0.05)、谷氨酰胺合成酶(GS)(1.720 fold VS H G group,P<0.05)等蛋白的表达水平。结论:模拟高糖环境可以影响视网膜Müller细胞谷氨酸转运体(GLAST)、谷氨酰胺合成酶的表达,其结局可能导致视神经细胞因谷氨酸堆积而导致的兴奋性毒性,白藜芦醇能提高Müller细胞谷氨酸转运体(GLAST)、谷氨酰胺合成酶表达,从而保护视神经细胞。

Objective： To investigate the effect of high glucose on glutamate transporter synthesis system on primary cultured SD rat retinal Mu ller cell （P7） and its possible mechanism. Methods： Primary cultured SD rat retinal M~ ller cell （PT） were treated and set as three groups： Control group, High glucose group, High glucose ＋ Resveratrol group. The expression of GLAST and GS were detected by western blot. Results： The stimulation of glucose decreased the expression of GLAST （0.225 fold vs. control, P〈0.05） and GS （0.653 fold, the VS control, P〈0.05）; resveratrol can significantly reverse the expression of GLAST （1.133 fold vs. the HG group, P〈0.05） and GS （1.720 fold vs. the HG the group, P〈0.05）. Conclusions： High glucose has effect on the expression of GLAST and GS in retinal Mu ller cells, and this may lead to optic nerve injury caused by excitatory glutamate accumulation. Resveratrol can protect the optic nerve by enhance the expression of GLAST and GS in retinal Mu ller cell.